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Regulation of T cell receptor delta gene rearrangement by c-Myb.

Publication ,  Journal Article
Hernández-Munain, C; Lauzurica, P; Krangel, MS
Published in: J Exp Med
January 1, 1996

Developmental activation of VDJ recombination at the T cell receptor (TCR) delta locus is controlled by an intronic transcriptional enhancer (E delta). Transcriptional activation by E delta is dependent on c-Myb. To determine whether c-Myb plays a role in the activation of TCR-delta gene rearrangement, we compared VDJ recombination in transgenic mice carrying two versions of a human TCR-delta gene minilocus recombination substrate. One includes a wild-type E delta, whereas the other carries an E delta with a mutation that abrogates c-Myb binding. We demonstrate that an intact Myb binding site is necessary for efficient rearrangement of the minilocus substrate, suggesting that c-Myb plays a crucial role in activating VDJ recombination at the endogenous TCR-delta locus.

Duke Scholars

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

January 1, 1996

Volume

183

Issue

1

Start / End Page

289 / 293

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Recombination, Genetic
  • Proto-Oncogene Proteins c-myb
  • Proto-Oncogene Proteins
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Immunology
  • Humans
  • Gene Rearrangement, delta-Chain T-Cell Antigen Receptor
 

Citation

APA
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ICMJE
MLA
NLM
Hernández-Munain, C., Lauzurica, P., & Krangel, M. S. (1996). Regulation of T cell receptor delta gene rearrangement by c-Myb. J Exp Med, 183(1), 289–293. https://doi.org/10.1084/jem.183.1.289
Hernández-Munain, C., P. Lauzurica, and M. S. Krangel. “Regulation of T cell receptor delta gene rearrangement by c-Myb.J Exp Med 183, no. 1 (January 1, 1996): 289–93. https://doi.org/10.1084/jem.183.1.289.
Hernández-Munain C, Lauzurica P, Krangel MS. Regulation of T cell receptor delta gene rearrangement by c-Myb. J Exp Med. 1996 Jan 1;183(1):289–93.
Hernández-Munain, C., et al. “Regulation of T cell receptor delta gene rearrangement by c-Myb.J Exp Med, vol. 183, no. 1, Jan. 1996, pp. 289–93. Pubmed, doi:10.1084/jem.183.1.289.
Hernández-Munain C, Lauzurica P, Krangel MS. Regulation of T cell receptor delta gene rearrangement by c-Myb. J Exp Med. 1996 Jan 1;183(1):289–293.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

January 1, 1996

Volume

183

Issue

1

Start / End Page

289 / 293

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Recombination, Genetic
  • Proto-Oncogene Proteins c-myb
  • Proto-Oncogene Proteins
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Immunology
  • Humans
  • Gene Rearrangement, delta-Chain T-Cell Antigen Receptor