Regulation of T cell receptor delta gene rearrangement by c-Myb.

Published

Journal Article

Developmental activation of VDJ recombination at the T cell receptor (TCR) delta locus is controlled by an intronic transcriptional enhancer (E delta). Transcriptional activation by E delta is dependent on c-Myb. To determine whether c-Myb plays a role in the activation of TCR-delta gene rearrangement, we compared VDJ recombination in transgenic mice carrying two versions of a human TCR-delta gene minilocus recombination substrate. One includes a wild-type E delta, whereas the other carries an E delta with a mutation that abrogates c-Myb binding. We demonstrate that an intact Myb binding site is necessary for efficient rearrangement of the minilocus substrate, suggesting that c-Myb plays a crucial role in activating VDJ recombination at the endogenous TCR-delta locus.

Full Text

Duke Authors

Cited Authors

  • Hernández-Munain, C; Lauzurica, P; Krangel, MS

Published Date

  • January 1, 1996

Published In

Volume / Issue

  • 183 / 1

Start / End Page

  • 289 - 293

PubMed ID

  • 8551234

Pubmed Central ID

  • 8551234

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.183.1.289

Language

  • eng

Conference Location

  • United States