An enhancer-blocking element between alpha and delta gene segments within the human T cell receptor alpha/delta locus.

Published

Journal Article

T cell receptor (TCR) alpha and delta gene segments are organized within a single genetic locus but are differentially regulated during T cell development. An enhancer-blocking element (BEAD-1, for blocking element alpha/delta 1) was localized to a 2.0-kb region 3' of TCR delta gene segments and 5' of TCR alpha joining gene segments within this locus. BEAD-1 blocked the ability of the TCR delta enhancer (Edelta) to activate a promoter when located between the two in a chromatin-integrated construct. We propose that BEAD-1 functions as a boundary that separates the TCR alpha/delta locus into distinct regulatory domains controlled by Edelta and the TCR alpha enhancer, and that it prevents Edelta from opening the chromatin of the TCR alpha joining gene segments for VDJ recombination at an early stage of T cell development.

Full Text

Duke Authors

Cited Authors

  • Zhong, XP; Krangel, MS

Published Date

  • May 13, 1997

Published In

Volume / Issue

  • 94 / 10

Start / End Page

  • 5219 - 5224

PubMed ID

  • 9144218

Pubmed Central ID

  • 9144218

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.10.5219

Language

  • eng

Conference Location

  • United States