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An enhancer-blocking element between alpha and delta gene segments within the human T cell receptor alpha/delta locus.

Publication ,  Journal Article
Zhong, XP; Krangel, MS
Published in: Proc Natl Acad Sci U S A
May 13, 1997

T cell receptor (TCR) alpha and delta gene segments are organized within a single genetic locus but are differentially regulated during T cell development. An enhancer-blocking element (BEAD-1, for blocking element alpha/delta 1) was localized to a 2.0-kb region 3' of TCR delta gene segments and 5' of TCR alpha joining gene segments within this locus. BEAD-1 blocked the ability of the TCR delta enhancer (Edelta) to activate a promoter when located between the two in a chromatin-integrated construct. We propose that BEAD-1 functions as a boundary that separates the TCR alpha/delta locus into distinct regulatory domains controlled by Edelta and the TCR alpha enhancer, and that it prevents Edelta from opening the chromatin of the TCR alpha joining gene segments for VDJ recombination at an early stage of T cell development.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

May 13, 1997

Volume

94

Issue

10

Start / End Page

5219 / 5224

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • Transfection
  • Transcription, Genetic
  • Transcription Factors
  • T-Lymphocytes
  • Restriction Mapping
  • Recombinant Fusion Proteins
  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Antigen, T-Cell, alpha-beta
  • Promoter Regions, Genetic
 

Citation

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Zhong, X. P., & Krangel, M. S. (1997). An enhancer-blocking element between alpha and delta gene segments within the human T cell receptor alpha/delta locus. Proc Natl Acad Sci U S A, 94(10), 5219–5224. https://doi.org/10.1073/pnas.94.10.5219
Zhong, X. P., and M. S. Krangel. “An enhancer-blocking element between alpha and delta gene segments within the human T cell receptor alpha/delta locus.Proc Natl Acad Sci U S A 94, no. 10 (May 13, 1997): 5219–24. https://doi.org/10.1073/pnas.94.10.5219.
Zhong, X. P., and M. S. Krangel. “An enhancer-blocking element between alpha and delta gene segments within the human T cell receptor alpha/delta locus.Proc Natl Acad Sci U S A, vol. 94, no. 10, May 1997, pp. 5219–24. Pubmed, doi:10.1073/pnas.94.10.5219.
Zhong XP, Krangel MS. An enhancer-blocking element between alpha and delta gene segments within the human T cell receptor alpha/delta locus. Proc Natl Acad Sci U S A. 1997 May 13;94(10):5219–5224.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

May 13, 1997

Volume

94

Issue

10

Start / End Page

5219 / 5224

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • Transfection
  • Transcription, Genetic
  • Transcription Factors
  • T-Lymphocytes
  • Restriction Mapping
  • Recombinant Fusion Proteins
  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Antigen, T-Cell, alpha-beta
  • Promoter Regions, Genetic