A developmental switch from TCR delta enhancer to TCR alpha enhancer function during thymocyte maturation.
Journal Article (Journal Article)
V(D)J recombination and transcription within the TCR alpha/delta locus are regulated by three characterized cis-acting elements: the TCR delta enhancer (Edelta), TCR alpha enhancer (Ealpha), and T early alpha (TEA) promoter. Analysis of enhancer and promoter occupancy and function in developing thymocytes in vivo indicates Edelta and Ealpha to be developmental-stage-specific enhancers, with Edelta "on" and Ealpha "off" in double-negative III thymocytes and Edelta "off" and Ealpha "on" in double-positive thymocytes. Edelta downregulation reflects a loss of occupancy. Surprisingly, Ealpha and TEA are extensively occupied even prior to activation. TCR delta downregulation in double-positive thymocytes depends on two events, Edelta inactivation and removal of TCR delta from the influence of Ealpha by chromosomal excision.
Full Text
Duke Authors
Cited Authors
- Hernández-Munain, C; Sleckman, BP; Krangel, MS
Published Date
- June 1999
Published In
Volume / Issue
- 10 / 6
Start / End Page
- 723 - 733
PubMed ID
- 10403647
International Standard Serial Number (ISSN)
- 1074-7613
Digital Object Identifier (DOI)
- 10.1016/s1074-7613(00)80071-0
Language
- eng
Conference Location
- United States