A developmental switch from TCR delta enhancer to TCR alpha enhancer function during thymocyte maturation.

Journal Article

V(D)J recombination and transcription within the TCR alpha/delta locus are regulated by three characterized cis-acting elements: the TCR delta enhancer (Edelta), TCR alpha enhancer (Ealpha), and T early alpha (TEA) promoter. Analysis of enhancer and promoter occupancy and function in developing thymocytes in vivo indicates Edelta and Ealpha to be developmental-stage-specific enhancers, with Edelta "on" and Ealpha "off" in double-negative III thymocytes and Edelta "off" and Ealpha "on" in double-positive thymocytes. Edelta downregulation reflects a loss of occupancy. Surprisingly, Ealpha and TEA are extensively occupied even prior to activation. TCR delta downregulation in double-positive thymocytes depends on two events, Edelta inactivation and removal of TCR delta from the influence of Ealpha by chromosomal excision.

Full Text

Duke Authors

Cited Authors

  • Hernández-Munain, C; Sleckman, BP; Krangel, MS

Published Date

  • June 1999

Published In

Volume / Issue

  • 10 / 6

Start / End Page

  • 723 - 733

PubMed ID

  • 10403647

International Standard Serial Number (ISSN)

  • 1074-7613

Language

  • eng

Conference Location

  • United States