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A developmental switch from TCR delta enhancer to TCR alpha enhancer function during thymocyte maturation.

Publication ,  Journal Article
Hernández-Munain, C; Sleckman, BP; Krangel, MS
Published in: Immunity
June 1999

V(D)J recombination and transcription within the TCR alpha/delta locus are regulated by three characterized cis-acting elements: the TCR delta enhancer (Edelta), TCR alpha enhancer (Ealpha), and T early alpha (TEA) promoter. Analysis of enhancer and promoter occupancy and function in developing thymocytes in vivo indicates Edelta and Ealpha to be developmental-stage-specific enhancers, with Edelta "on" and Ealpha "off" in double-negative III thymocytes and Edelta "off" and Ealpha "on" in double-positive thymocytes. Edelta downregulation reflects a loss of occupancy. Surprisingly, Ealpha and TEA are extensively occupied even prior to activation. TCR delta downregulation in double-positive thymocytes depends on two events, Edelta inactivation and removal of TCR delta from the influence of Ealpha by chromosomal excision.

Duke Scholars

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

June 1999

Volume

10

Issue

6

Start / End Page

723 / 733

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocyte Subsets
  • Recombination, Genetic
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Immunology
  • Genes, T-Cell Receptor delta
  • Genes, T-Cell Receptor alpha
 

Citation

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Chicago
ICMJE
MLA
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Hernández-Munain, C., Sleckman, B. P., & Krangel, M. S. (1999). A developmental switch from TCR delta enhancer to TCR alpha enhancer function during thymocyte maturation. Immunity, 10(6), 723–733. https://doi.org/10.1016/s1074-7613(00)80071-0
Hernández-Munain, C., B. P. Sleckman, and M. S. Krangel. “A developmental switch from TCR delta enhancer to TCR alpha enhancer function during thymocyte maturation.Immunity 10, no. 6 (June 1999): 723–33. https://doi.org/10.1016/s1074-7613(00)80071-0.
Hernández-Munain C, Sleckman BP, Krangel MS. A developmental switch from TCR delta enhancer to TCR alpha enhancer function during thymocyte maturation. Immunity. 1999 Jun;10(6):723–33.
Hernández-Munain, C., et al. “A developmental switch from TCR delta enhancer to TCR alpha enhancer function during thymocyte maturation.Immunity, vol. 10, no. 6, June 1999, pp. 723–33. Pubmed, doi:10.1016/s1074-7613(00)80071-0.
Hernández-Munain C, Sleckman BP, Krangel MS. A developmental switch from TCR delta enhancer to TCR alpha enhancer function during thymocyte maturation. Immunity. 1999 Jun;10(6):723–733.
Journal cover image

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

June 1999

Volume

10

Issue

6

Start / End Page

723 / 733

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocyte Subsets
  • Recombination, Genetic
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Immunology
  • Genes, T-Cell Receptor delta
  • Genes, T-Cell Receptor alpha