Scholars@Duke publication: A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion.

A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion.

Publication , Journal Article

Tripathi, R; Jackson, A; Krangel, MS

Published in: J Immunol

March 1, 2002

To investigate chromatin control of TCR beta rearrangement and allelic exclusion, we analyzed TCR beta chromatin structure in double negative (DN) thymocytes, which are permissive for TCR beta recombination, and in double positive (DP) thymocytes, which are postallelic exclusion and nonpermissive for Vbeta to DbetaJbeta recombination. Histone acetylation mapping and DNase I sensitivity studies indicate Vbeta and DbetaJbeta segments to be hyperacetylated and accessible in DN thymocytes. However, they are separated from each other by hypoacetylated and inaccessible trypsinogen chromatin. The transition from DN to DP is accompanied by selective down-regulation of Vbeta acetylation and accessibility. The level of DP acetylation and accessibility is minimal for five of six Vbeta segments studied but remains substantial for one. Hence, the observed changes in Vbeta chromatin structure appear sufficient to account for allelic exclusion of many Vbeta segments. They may contribute to, but not by themselves fully account for, allelic exclusion of others.

Duke Scholars

Author Annette M Jackson Surgery, Abdominal Transplant Surgery

Author Michael S. Krangel Integrative Immunobiology

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Published In

J Immunol

DOI

10.4049/jimmunol.168.5.2316

ISSN

0022-1767

Publication Date

March 1, 2002

Volume

168

Issue

Start / End Page

2316 / 2324

Location

United States

Related Subject Headings

Trypsinogen
Thymus Gland
T-Lymphocytes
Precipitin Tests
Models, Genetic
Immunology
Immunoglobulin Variable Region
Histones
Genes, T-Cell Receptor beta
Gene Rearrangement, beta-Chain T-Cell Antigen Receptor

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Tripathi, R., Jackson, A., & Krangel, M. S. (2002). A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion. J Immunol, 168(5), 2316–2324. https://doi.org/10.4049/jimmunol.168.5.2316

Tripathi, Rajkamal, Annette Jackson, and Michael S. Krangel. “A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion.” J Immunol 168, no. 5 (March 1, 2002): 2316–24. https://doi.org/10.4049/jimmunol.168.5.2316.

Tripathi R, Jackson A, Krangel MS. A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion. J Immunol. 2002 Mar 1;168(5):2316–24.

Tripathi, Rajkamal, et al. “A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion.” J Immunol, vol. 168, no. 5, Mar. 2002, pp. 2316–24. Pubmed, doi:10.4049/jimmunol.168.5.2316.

Tripathi R, Jackson A, Krangel MS. A change in the structure of Vbeta chromatin associated with TCR beta allelic exclusion. J Immunol. 2002 Mar 1;168(5):2316–2324.