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Expression of multiple chemokine genes by a human mast cell leukemia.

Publication ,  Journal Article
Selvan, RS; Butterfield, JH; Krangel, MS
Published in: J Biol Chem
May 13, 1994

The chemokines are a large group of cytokines that are recognized to be important mediators of inflammation. In this study we show that the human mast cell leukemia line HMC-1 is a source of multiple chemokines, including I-309, monocyte chemoattractant protein 1, macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, RANTES, and interleukin-8. I-309 and MCP-1 transcripts are expressed at low levels in unstimulated HMC-1. However, phorbol ester treatment up-regulates these and other chemokine transcript levels and also up-regulates chemokine protein synthesis and secretion. Induction of chemokine transcripts in HMC-1 requires de novo protein synthesis. We compared the effects of anti-inflammatory glucocorticoids on the expression of chemokine genes in HMC-1 to their effects in activated T-cells. We find that methyl-prednisolone reduces MCP-1 but not other chemokine transcripts in HMC-1, even though there are distinct and more general effects on chemokine transcripts in activated T-cells. These effects are attributed to inhibition of transcription rather than transcript stability. Our results suggest that human mast cells may be a source of multiple chemokines, that glucocorticoids may inhibit the expression of only a subset of these chemokines, and that mast cells and T-cell chemokine expression may occur via distinct regulatory pathways.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

May 13, 1994

Volume

269

Issue

19

Start / End Page

13893 / 13898

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes
  • RNA, Messenger
  • Mast Cells
  • Lymphocyte Activation
  • Leukemia, Mast-Cell
  • Humans
  • Glucocorticoids
  • Gene Expression Regulation, Neoplastic
  • Cytokines
 

Citation

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MLA
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Selvan, R. S., Butterfield, J. H., & Krangel, M. S. (1994). Expression of multiple chemokine genes by a human mast cell leukemia. J Biol Chem, 269(19), 13893–13898.
Selvan, R. S., J. H. Butterfield, and M. S. Krangel. “Expression of multiple chemokine genes by a human mast cell leukemia.J Biol Chem 269, no. 19 (May 13, 1994): 13893–98.
Selvan RS, Butterfield JH, Krangel MS. Expression of multiple chemokine genes by a human mast cell leukemia. J Biol Chem. 1994 May 13;269(19):13893–8.
Selvan, R. S., et al. “Expression of multiple chemokine genes by a human mast cell leukemia.J Biol Chem, vol. 269, no. 19, May 1994, pp. 13893–98.
Selvan RS, Butterfield JH, Krangel MS. Expression of multiple chemokine genes by a human mast cell leukemia. J Biol Chem. 1994 May 13;269(19):13893–13898.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

May 13, 1994

Volume

269

Issue

19

Start / End Page

13893 / 13898

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes
  • RNA, Messenger
  • Mast Cells
  • Lymphocyte Activation
  • Leukemia, Mast-Cell
  • Humans
  • Glucocorticoids
  • Gene Expression Regulation, Neoplastic
  • Cytokines