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Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.

Publication ,  Journal Article
Sikes, ML; Meade, A; Tripathi, R; Krangel, MS; Oltz, EM
Published in: Proc Natl Acad Sci U S A
September 17, 2002

Antigen receptor gene assembly is regulated by transcriptional promoters and enhancers, which control the accessibility of gene segments to a lymphocyte-specific V(D)J recombinase. However, it remained unclear whether accessibility depends on the process of transcription itself or chromatin modifications that accompany transcription. By using T cell receptor beta substrates that integrate stably into nuclear chromatin, we show that promoter location, rather than germ-line transcription or histone acetylation, is a primary determinant of recombination efficiency. These spatial constraints on promoter positioning may reflect an RNA polymerase-independent mechanism to target adjacent gene segments for chromatin remodeling events that facilitate rearrangement.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 17, 2002

Volume

99

Issue

19

Start / End Page

12309 / 12314

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • Transfection
  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, alpha-beta
  • Promoter Regions, Genetic
  • Nuclear Proteins
  • Models, Genetic
  • Lymphocytes
  • Humans
  • Homeodomain Proteins
 

Citation

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Sikes, M. L., Meade, A., Tripathi, R., Krangel, M. S., & Oltz, E. M. (2002). Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility. Proc Natl Acad Sci U S A, 99(19), 12309–12314. https://doi.org/10.1073/pnas.182166699
Sikes, Michael L., Amber Meade, Rajkamal Tripathi, Michael S. Krangel, and Eugene M. Oltz. “Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.Proc Natl Acad Sci U S A 99, no. 19 (September 17, 2002): 12309–14. https://doi.org/10.1073/pnas.182166699.
Sikes ML, Meade A, Tripathi R, Krangel MS, Oltz EM. Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility. Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12309–14.
Sikes, Michael L., et al. “Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.Proc Natl Acad Sci U S A, vol. 99, no. 19, Sept. 2002, pp. 12309–14. Pubmed, doi:10.1073/pnas.182166699.
Sikes ML, Meade A, Tripathi R, Krangel MS, Oltz EM. Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility. Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12309–12314.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 17, 2002

Volume

99

Issue

19

Start / End Page

12309 / 12314

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • Transfection
  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, alpha-beta
  • Promoter Regions, Genetic
  • Nuclear Proteins
  • Models, Genetic
  • Lymphocytes
  • Humans
  • Homeodomain Proteins