Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.
Journal Article (Journal Article)
Antigen receptor gene assembly is regulated by transcriptional promoters and enhancers, which control the accessibility of gene segments to a lymphocyte-specific V(D)J recombinase. However, it remained unclear whether accessibility depends on the process of transcription itself or chromatin modifications that accompany transcription. By using T cell receptor beta substrates that integrate stably into nuclear chromatin, we show that promoter location, rather than germ-line transcription or histone acetylation, is a primary determinant of recombination efficiency. These spatial constraints on promoter positioning may reflect an RNA polymerase-independent mechanism to target adjacent gene segments for chromatin remodeling events that facilitate rearrangement.
Full Text
Duke Authors
Cited Authors
- Sikes, ML; Meade, A; Tripathi, R; Krangel, MS; Oltz, EM
Published Date
- September 17, 2002
Published In
Volume / Issue
- 99 / 19
Start / End Page
- 12309 - 12314
PubMed ID
- 12196630
Pubmed Central ID
- PMC129441
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.182166699
Language
- eng
Conference Location
- United States