Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.


Journal Article

Antigen receptor gene assembly is regulated by transcriptional promoters and enhancers, which control the accessibility of gene segments to a lymphocyte-specific V(D)J recombinase. However, it remained unclear whether accessibility depends on the process of transcription itself or chromatin modifications that accompany transcription. By using T cell receptor beta substrates that integrate stably into nuclear chromatin, we show that promoter location, rather than germ-line transcription or histone acetylation, is a primary determinant of recombination efficiency. These spatial constraints on promoter positioning may reflect an RNA polymerase-independent mechanism to target adjacent gene segments for chromatin remodeling events that facilitate rearrangement.

Full Text

Duke Authors

Cited Authors

  • Sikes, ML; Meade, A; Tripathi, R; Krangel, MS; Oltz, EM

Published Date

  • September 17, 2002

Published In

Volume / Issue

  • 99 / 19

Start / End Page

  • 12309 - 12314

PubMed ID

  • 12196630

Pubmed Central ID

  • 12196630

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.182166699


  • eng

Conference Location

  • United States