Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.

Published

Journal Article

Antigen receptor gene assembly is regulated by transcriptional promoters and enhancers, which control the accessibility of gene segments to a lymphocyte-specific V(D)J recombinase. However, it remained unclear whether accessibility depends on the process of transcription itself or chromatin modifications that accompany transcription. By using T cell receptor beta substrates that integrate stably into nuclear chromatin, we show that promoter location, rather than germ-line transcription or histone acetylation, is a primary determinant of recombination efficiency. These spatial constraints on promoter positioning may reflect an RNA polymerase-independent mechanism to target adjacent gene segments for chromatin remodeling events that facilitate rearrangement.

Full Text

Duke Authors

Cited Authors

  • Sikes, ML; Meade, A; Tripathi, R; Krangel, MS; Oltz, EM

Published Date

  • September 2002

Published In

Volume / Issue

  • 99 / 19

Start / End Page

  • 12309 - 12314

PubMed ID

  • 12196630

Pubmed Central ID

  • 12196630

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.182166699

Language

  • eng