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Secretion of HLA-A and -B antigens via an alternative RNA splicing pathway.

Publication ,  Journal Article
Krangel, MS
Published in: J Exp Med
May 1, 1986

Human class I major histocompatibility antigens (HLA-A, -B and -C) are integral membrane protein heterodimers, which are anchored in the membrane via a stretch of hydrophobic amino acids near the carboxyl terminus of the heavy chain. It has previously been shown that a mutagenized cell line secretes a water soluble form of the HLA-A2 antigen, due to a pattern of RNA splicing that removes exon 5 (encoding the transmembrane hydrophobic amino acids) from mature, HLA-A2--encoding transcripts. The present study was undertaken to assess whether a similar process might be operative in nonmutagenized cells. It is shown that water soluble class I molecules (primarily HLA-A24) are secreted by the T leukemic cell line HPB-ALL, and that alternative splicing removes exon 5 from a fraction of HLA-A24--encoding transcripts. It is further shown that class I molecules are secreted, possibly in an allele-specific fashion, from a variety of tumor cells and normal cells. The possible relationship between these findings and previous reports of HLA-A and -B antigens in human serum is discussed.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

May 1, 1986

Volume

163

Issue

5

Start / End Page

1173 / 1190

Location

United States

Related Subject Headings

  • Solubility
  • RNA Splicing
  • Molecular Weight
  • Membrane Proteins
  • Lymphocytes
  • Liver Neoplasms, Experimental
  • Isoelectric Point
  • Immunology
  • Humans
  • HLA Antigens
 

Citation

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Krangel, M. S. (1986). Secretion of HLA-A and -B antigens via an alternative RNA splicing pathway. J Exp Med, 163(5), 1173–1190. https://doi.org/10.1084/jem.163.5.1173
Krangel, M. S. “Secretion of HLA-A and -B antigens via an alternative RNA splicing pathway.J Exp Med 163, no. 5 (May 1, 1986): 1173–90. https://doi.org/10.1084/jem.163.5.1173.
Krangel MS. Secretion of HLA-A and -B antigens via an alternative RNA splicing pathway. J Exp Med. 1986 May 1;163(5):1173–90.
Krangel, M. S. “Secretion of HLA-A and -B antigens via an alternative RNA splicing pathway.J Exp Med, vol. 163, no. 5, May 1986, pp. 1173–90. Pubmed, doi:10.1084/jem.163.5.1173.
Krangel MS. Secretion of HLA-A and -B antigens via an alternative RNA splicing pathway. J Exp Med. 1986 May 1;163(5):1173–1190.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

May 1, 1986

Volume

163

Issue

5

Start / End Page

1173 / 1190

Location

United States

Related Subject Headings

  • Solubility
  • RNA Splicing
  • Molecular Weight
  • Membrane Proteins
  • Lymphocytes
  • Liver Neoplasms, Experimental
  • Isoelectric Point
  • Immunology
  • Humans
  • HLA Antigens