Characterization of a B lymphoblastoid cell line mutant that secretes HLA-A2.

Journal Article (Journal Article)

HLA-A2 antigen mutants were obtained previously from the B lymphoblastoid cell line T5-1 by mutagenesis followed by immunoselection. Here we present biochemical studies of one particular mutant, clone 8.14.1. These cells synthesize two forms of HLA-A2: a minor form, which remains cell-associated at all times, and an abundant form, which is secreted. The former appears by SDS-PAGE to be slightly larger than T5-1 HLA-A2, whereas the latter appears to be 4000 to 5000 daltons smaller. In vitro translation and in vivo pulse-chase studies suggest that these species are not related to each other by post-translational processing. Proteolytic digestion studies localize the resulting structural alteration in the mobility difference between wild-type and secreted HLA-A2 to a region near the carboxy terminus of the HLA-A2 heavy chain; however, their extreme carboxy termini appear similar, if not identical. We suggest that the secreted form may result from a pattern of RNA splicing in which the exon encoding the hydrophobic, membrane-spanning region is frequently deleted.

Full Text

Duke Authors

Cited Authors

  • Krangel, MS; Pious, D; Strominger, JL

Published Date

  • June 1984

Published In

Volume / Issue

  • 132 / 6

Start / End Page

  • 2984 - 2991

PubMed ID

  • 6609965

International Standard Serial Number (ISSN)

  • 0022-1767


  • eng

Conference Location

  • United States