Immunochemical proof that a novel rearranging gene encodes the T cell receptor delta subunit.

Published

Journal Article

The T cell receptor (TCR) delta protein is expressed as part of a heterodimer with TCR gamma, in association with the CD3 polypeptides on a subset of functional peripheral blood T lymphocytes, thymocytes, and certain leukemic T cell lines. A monoclonal antibody directed against TCR delta was produced that binds specifically to the surface of several TCR gamma delta cell lines and immunoprecipitates the TCR gamma delta as a heterodimer from Triton X-100 detergent lysates and also immunoprecipitates the TCR delta subunit alone after chain separation. A candidate human TCR delta complementary DNA clone (IDP2 O-240/38), reported in a companion paper, was isolated by the subtractive library approach from a TCR gamma delta cell line. This complementary DNA clone was used to direct the synthesis of a polypeptide that is specifically recognized by the monoclonal antibody to TCR delta. This complementary DNA clone thus corresponds to the gene that encodes the TCR delta subunit.

Full Text

Duke Authors

Cited Authors

  • Band, H; Hochstenbach, F; McLean, J; Hata, S; Krangel, MS; Brenner, MB

Published Date

  • October 1, 1987

Published In

Volume / Issue

  • 238 / 4827

Start / End Page

  • 682 - 684

PubMed ID

  • 3672118

Pubmed Central ID

  • 3672118

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.3672118

Language

  • eng