A novel polypeptide secreted by activated human T lymphocytes.

Journal Article

We have identified two cDNA clones, I-309 and G-26, which define genes expressed abundantly in activated human PBMC, but at low or undetectable levels in resting PBMC. Based upon nucleotide sequence analysis, both clones are predicted to encode small, structurally related polypeptides, each containing a hydrophobic leader sequence characteristic of secreted proteins and a motif of four conserved cysteine residues. Further, I-309 and G-26 are structurally related to a growing family of genes that apparently encode small polypeptides whose secretion is induced upon cell activation. I-309 represents a previously undescribed human gene. We have generated an anti-peptide antiserum to the I-309 gene product which recognizes proteins in culture supernatants of an activated T cell clone and of COS cells transfected with the I-309 cDNA, supporting the idea that I-309 encodes a secreted protein. Because I-309 encodes a small protein secreted by activated T cells that displays structural features similar to other cytokines, we believe that it defines a novel cytokine with as yet unknown function.

Full Text

Duke Authors

Cited Authors

  • Miller, MD; Hata, S; De Waal Malefyt, R; Krangel, MS

Published Date

  • November 1, 1989

Published In

Volume / Issue

  • 143 / 9

Start / End Page

  • 2907 - 2916

PubMed ID

  • 2809212

International Standard Serial Number (ISSN)

  • 0022-1767

Language

  • eng

Conference Location

  • United States