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A novel polypeptide secreted by activated human T lymphocytes.

Publication ,  Journal Article
Miller, MD; Hata, S; De Waal Malefyt, R; Krangel, MS
Published in: J Immunol
November 1, 1989

We have identified two cDNA clones, I-309 and G-26, which define genes expressed abundantly in activated human PBMC, but at low or undetectable levels in resting PBMC. Based upon nucleotide sequence analysis, both clones are predicted to encode small, structurally related polypeptides, each containing a hydrophobic leader sequence characteristic of secreted proteins and a motif of four conserved cysteine residues. Further, I-309 and G-26 are structurally related to a growing family of genes that apparently encode small polypeptides whose secretion is induced upon cell activation. I-309 represents a previously undescribed human gene. We have generated an anti-peptide antiserum to the I-309 gene product which recognizes proteins in culture supernatants of an activated T cell clone and of COS cells transfected with the I-309 cDNA, supporting the idea that I-309 encodes a secreted protein. Because I-309 encodes a small protein secreted by activated T cells that displays structural features similar to other cytokines, we believe that it defines a novel cytokine with as yet unknown function.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

November 1, 1989

Volume

143

Issue

9

Start / End Page

2907 / 2916

Location

United States

Related Subject Headings

  • Time Factors
  • T-Lymphocytes
  • RNA, Messenger
  • Multigene Family
  • Molecular Weight
  • Molecular Sequence Data
  • Lymphokines
  • Lymphocyte Activation
  • Immunology
  • Humans
 

Citation

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Miller, M. D., Hata, S., De Waal Malefyt, R., & Krangel, M. S. (1989). A novel polypeptide secreted by activated human T lymphocytes. J Immunol, 143(9), 2907–2916.
Miller, M. D., S. Hata, R. De Waal Malefyt, and M. S. Krangel. “A novel polypeptide secreted by activated human T lymphocytes.J Immunol 143, no. 9 (November 1, 1989): 2907–16.
Miller MD, Hata S, De Waal Malefyt R, Krangel MS. A novel polypeptide secreted by activated human T lymphocytes. J Immunol. 1989 Nov 1;143(9):2907–16.
Miller, M. D., et al. “A novel polypeptide secreted by activated human T lymphocytes.J Immunol, vol. 143, no. 9, Nov. 1989, pp. 2907–16.
Miller MD, Hata S, De Waal Malefyt R, Krangel MS. A novel polypeptide secreted by activated human T lymphocytes. J Immunol. 1989 Nov 1;143(9):2907–2916.

Published In

J Immunol

ISSN

0022-1767

Publication Date

November 1, 1989

Volume

143

Issue

9

Start / End Page

2907 / 2916

Location

United States

Related Subject Headings

  • Time Factors
  • T-Lymphocytes
  • RNA, Messenger
  • Multigene Family
  • Molecular Weight
  • Molecular Sequence Data
  • Lymphokines
  • Lymphocyte Activation
  • Immunology
  • Humans