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Regulation of T cell receptor alpha gene assembly by a complex hierarchy of germline Jalpha promoters.

Publication ,  Journal Article
Hawwari, A; Bock, C; Krangel, MS
Published in: Nat Immunol
May 2005

Assembly of the gene encoding T cell receptor alpha (Tcra) is characterized by an orderly progression of primary and secondary V(alpha)-to-J(alpha) recombination events across the J(alpha) array, but the targeting mechanisms responsible for this progression are mostly unknown. Studies have shown that the T early-alpha promoter is important in targeting primary Tcra rearrangements. We found that T early-alpha and a previously unknown promoter associated with J(alpha)49 targeted primary recombination to discrete sets of constant alpha region (C(alpha))-distal J(alpha) segments and together directed nearly all normal primary recombination events. Furthermore, deletion of the T early-alpha promoter activated previously suppressed downstream promoters and stimulated primary rearrangement to centrally located J(alpha) segments. Central promoter derepression also occurred after primary rearrangement, thereby providing a mechanism to target secondary recombination events.

Duke Scholars

Published In

Nat Immunol

DOI

ISSN

1529-2908

Publication Date

May 2005

Volume

6

Issue

5

Start / End Page

481 / 489

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • T-Lymphocytes
  • Sequence Deletion
  • Receptors, Antigen, T-Cell, alpha-beta
  • Promoter Regions, Genetic
  • Mice
  • Immunology
  • Humans
  • Histones
  • Germ Cells
 

Citation

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Hawwari, A., Bock, C., & Krangel, M. S. (2005). Regulation of T cell receptor alpha gene assembly by a complex hierarchy of germline Jalpha promoters. Nat Immunol, 6(5), 481–489. https://doi.org/10.1038/ni1189
Hawwari, Abbas, Cheryl Bock, and Michael S. Krangel. “Regulation of T cell receptor alpha gene assembly by a complex hierarchy of germline Jalpha promoters.Nat Immunol 6, no. 5 (May 2005): 481–89. https://doi.org/10.1038/ni1189.
Hawwari, Abbas, et al. “Regulation of T cell receptor alpha gene assembly by a complex hierarchy of germline Jalpha promoters.Nat Immunol, vol. 6, no. 5, May 2005, pp. 481–89. Pubmed, doi:10.1038/ni1189.
Hawwari A, Bock C, Krangel MS. Regulation of T cell receptor alpha gene assembly by a complex hierarchy of germline Jalpha promoters. Nat Immunol. 2005 May;6(5):481–489.

Published In

Nat Immunol

DOI

ISSN

1529-2908

Publication Date

May 2005

Volume

6

Issue

5

Start / End Page

481 / 489

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • T-Lymphocytes
  • Sequence Deletion
  • Receptors, Antigen, T-Cell, alpha-beta
  • Promoter Regions, Genetic
  • Mice
  • Immunology
  • Humans
  • Histones
  • Germ Cells