Regulation of T cell receptor alpha gene assembly by a complex hierarchy of germline Jalpha promoters.

Published

Journal Article

Assembly of the gene encoding T cell receptor alpha (Tcra) is characterized by an orderly progression of primary and secondary V(alpha)-to-J(alpha) recombination events across the J(alpha) array, but the targeting mechanisms responsible for this progression are mostly unknown. Studies have shown that the T early-alpha promoter is important in targeting primary Tcra rearrangements. We found that T early-alpha and a previously unknown promoter associated with J(alpha)49 targeted primary recombination to discrete sets of constant alpha region (C(alpha))-distal J(alpha) segments and together directed nearly all normal primary recombination events. Furthermore, deletion of the T early-alpha promoter activated previously suppressed downstream promoters and stimulated primary rearrangement to centrally located J(alpha) segments. Central promoter derepression also occurred after primary rearrangement, thereby providing a mechanism to target secondary recombination events.

Full Text

Duke Authors

Cited Authors

  • Hawwari, A; Bock, C; Krangel, MS

Published Date

  • May 2005

Published In

Volume / Issue

  • 6 / 5

Start / End Page

  • 481 - 489

PubMed ID

  • 15806105

Pubmed Central ID

  • 15806105

International Standard Serial Number (ISSN)

  • 1529-2908

Digital Object Identifier (DOI)

  • 10.1038/ni1189

Language

  • eng

Conference Location

  • United States