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Regulation of the murine Ddelta2 promoter by upstream stimulatory factor 1, Runx1, and c-Myb.

Publication ,  Journal Article
Carabana, J; Ortigoza, E; Krangel, MS
Published in: J Immunol
April 1, 2005

Accessibility control of V(D)J recombination at Ag receptor loci depends on the coordinate activities of transcriptional enhancers and germline promoters. Recombination of murine Tcrd gene segments is known to be regulated, at least in part, by the Tcrd enhancer (Edelta) situated in the Jdelta2-Cdelta intron. However, there has been little characterization of promoters and other cis-acting elements that are activated by or collaborate with Edelta and that might function to regulate Tcrd gene recombination events. We now describe a strong promoter that is tightly associated with the murine Ddelta2 gene segment. EMSAs reveal that upstream stimulatory factor 1, Runx1, c-Myb, lymphoid enhancer binding factor 1, NF1, and E47 all interact with this promoter in vitro. Of these, upstream stimulatory factor 1, Runx1, and c-Myb appear necessary for full promoter activity in transiently transfected cells. Moreover, the same three factors were found to interact with the promoter in vivo by chromatin immunoprecipitation. We suggest that these factors play important roles as Edelta-dependent regulators of Ddelta2 accessibility in vivo. Consistent with the established roles of c-Myb and Runx factors in Edelta function, we detected low level, enhancer-independent activity of the Ddelta2 promoter in transient transfection experiments. We speculate that the Ddelta2 promoter may play a role as a weak, enhancer-independent regulator in vivo, and might contribute to residual Tcrd rearrangement in Edelta(-/-) mice.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

April 1, 2005

Volume

174

Issue

7

Start / End Page

4144 / 4152

Location

United States

Related Subject Headings

  • Upstream Stimulatory Factors
  • Transcription Factors
  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, gamma-delta
  • Proto-Oncogene Proteins c-myb
  • Proto-Oncogene Proteins
  • Promoter Regions, Genetic
  • Mice
  • Immunology
  • Gene Rearrangement, delta-Chain T-Cell Antigen Receptor
 

Citation

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Carabana, J., Ortigoza, E., & Krangel, M. S. (2005). Regulation of the murine Ddelta2 promoter by upstream stimulatory factor 1, Runx1, and c-Myb. J Immunol, 174(7), 4144–4152. https://doi.org/10.4049/jimmunol.174.7.4144
Carabana, Juan, Eric Ortigoza, and Michael S. Krangel. “Regulation of the murine Ddelta2 promoter by upstream stimulatory factor 1, Runx1, and c-Myb.J Immunol 174, no. 7 (April 1, 2005): 4144–52. https://doi.org/10.4049/jimmunol.174.7.4144.
Carabana J, Ortigoza E, Krangel MS. Regulation of the murine Ddelta2 promoter by upstream stimulatory factor 1, Runx1, and c-Myb. J Immunol. 2005 Apr 1;174(7):4144–52.
Carabana, Juan, et al. “Regulation of the murine Ddelta2 promoter by upstream stimulatory factor 1, Runx1, and c-Myb.J Immunol, vol. 174, no. 7, Apr. 2005, pp. 4144–52. Pubmed, doi:10.4049/jimmunol.174.7.4144.
Carabana J, Ortigoza E, Krangel MS. Regulation of the murine Ddelta2 promoter by upstream stimulatory factor 1, Runx1, and c-Myb. J Immunol. 2005 Apr 1;174(7):4144–4152.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

April 1, 2005

Volume

174

Issue

7

Start / End Page

4144 / 4152

Location

United States

Related Subject Headings

  • Upstream Stimulatory Factors
  • Transcription Factors
  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, gamma-delta
  • Proto-Oncogene Proteins c-myb
  • Proto-Oncogene Proteins
  • Promoter Regions, Genetic
  • Mice
  • Immunology
  • Gene Rearrangement, delta-Chain T-Cell Antigen Receptor