Regulation of T cell receptor-alpha gene recombination by transcription.

Published

Journal Article

Despite the longstanding correlation between transcription and variable-(diversity)-joining (V(D)J) recombination, it is unknown whether transcription itself can direct recombinase targeting. Here we show that blockade of transcriptional elongation through the mouse T cell receptor-alpha (Tcra) locus suppressed V(alpha)-to-J(alpha) recombination and chromatin remodeling of J(alpha) segments. Transcriptional blockade also derepressed cryptic J(alpha) promoters. Our results demonstrate two key functions for transcription in Tcra locus regulation. Transcription increases the recombination of J(alpha) segments located within several kilobases of a promoter and prevents the activation of downstream promoters through transcriptional interference. These influences promote an ordered progression of Tcra locus recombination events and selection of a robust Tcra repertoire.

Full Text

Duke Authors

Cited Authors

  • Abarrategui, I; Krangel, MS

Published Date

  • October 2006

Published In

Volume / Issue

  • 7 / 10

Start / End Page

  • 1109 - 1115

PubMed ID

  • 16936730

Pubmed Central ID

  • 16936730

International Standard Serial Number (ISSN)

  • 1529-2908

Digital Object Identifier (DOI)

  • 10.1038/ni1379

Language

  • eng

Conference Location

  • United States