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Regulation of T cell receptor-alpha gene recombination by transcription.

Publication ,  Journal Article
Abarrategui, I; Krangel, MS
Published in: Nat Immunol
October 2006

Despite the longstanding correlation between transcription and variable-(diversity)-joining (V(D)J) recombination, it is unknown whether transcription itself can direct recombinase targeting. Here we show that blockade of transcriptional elongation through the mouse T cell receptor-alpha (Tcra) locus suppressed V(alpha)-to-J(alpha) recombination and chromatin remodeling of J(alpha) segments. Transcriptional blockade also derepressed cryptic J(alpha) promoters. Our results demonstrate two key functions for transcription in Tcra locus regulation. Transcription increases the recombination of J(alpha) segments located within several kilobases of a promoter and prevents the activation of downstream promoters through transcriptional interference. These influences promote an ordered progression of Tcra locus recombination events and selection of a robust Tcra repertoire.

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Published In

Nat Immunol

DOI

ISSN

1529-2908

Publication Date

October 2006

Volume

7

Issue

10

Start / End Page

1109 / 1115

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Sequence Deletion
  • Recombination, Genetic
  • Promoter Regions, Genetic
  • Mice, Mutant Strains
  • Mice
  • Immunology
  • Immunoglobulin Joining Region
  • Genes, T-Cell Receptor alpha
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
 

Citation

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MLA
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Abarrategui, I., & Krangel, M. S. (2006). Regulation of T cell receptor-alpha gene recombination by transcription. Nat Immunol, 7(10), 1109–1115. https://doi.org/10.1038/ni1379
Abarrategui, Iratxe, and Michael S. Krangel. “Regulation of T cell receptor-alpha gene recombination by transcription.Nat Immunol 7, no. 10 (October 2006): 1109–15. https://doi.org/10.1038/ni1379.
Abarrategui I, Krangel MS. Regulation of T cell receptor-alpha gene recombination by transcription. Nat Immunol. 2006 Oct;7(10):1109–15.
Abarrategui, Iratxe, and Michael S. Krangel. “Regulation of T cell receptor-alpha gene recombination by transcription.Nat Immunol, vol. 7, no. 10, Oct. 2006, pp. 1109–15. Pubmed, doi:10.1038/ni1379.
Abarrategui I, Krangel MS. Regulation of T cell receptor-alpha gene recombination by transcription. Nat Immunol. 2006 Oct;7(10):1109–1115.

Published In

Nat Immunol

DOI

ISSN

1529-2908

Publication Date

October 2006

Volume

7

Issue

10

Start / End Page

1109 / 1115

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Sequence Deletion
  • Recombination, Genetic
  • Promoter Regions, Genetic
  • Mice, Mutant Strains
  • Mice
  • Immunology
  • Immunoglobulin Joining Region
  • Genes, T-Cell Receptor alpha
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor