Histamine stimulates calcium-mediated protein phosphorylation in a colonic epithelial cell line.


Journal Article

Protein phosphorylation responses in intact enterocytes were examined by stimulating 32Pi-labeled T84 cell monolayers with histamine and resolving proteins by two-dimensional gel electrophoresis. Histamine increases 32P-incorporation into two acidic proteins of Mr 83,000 and of Mr 29,000, designated p83 and p29. Labeling of p83 and p29 is also increased in cells exposed to ionomycin, but not in cells exposed to vasoactive intestinal peptide under conditions resulting in cAMP-mediated secretion and cAMP-stimulated protein phosphorylation. When T84 cell fractions are incubated with [gamma-32P]ATP, labeling of p83 is stimulated by Ca++, but not by cAMP. Thus, histamine stimulates Ca++-mediated protein phosphorylation during the regulation of Cl- secretion.

Full Text

Duke Authors

Cited Authors

  • Cohn, JA; Dougherty, NC; King, WF

Published Date

  • December 15, 1989

Published In

Volume / Issue

  • 165 / 2

Start / End Page

  • 810 - 816

PubMed ID

  • 2557021

Pubmed Central ID

  • 2557021

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/s0006-291x(89)80038-5


  • eng

Conference Location

  • United States