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Phosphatidic acid modulates Cl- secretion in T84 cells: varying effects depending on mode of stimulation.

Publication ,  Journal Article
Vajanaphanich, M; Kachintorn, U; Barrett, KE; Cohn, JA; Dharmsathaphorn, K; Traynor-Kaplan, A
Published in: Am J Physiol
May 1993

Cl- secretion in T84 cells evoked by a stimulus that activates protein kinase C, carbachol, was associated with elevated levels of 32P-labeled phosphatidic acid (PA). PA's role in the regulation of Cl- secretion was explored by examining the effect of exogenous PA (10(-4) M) on Cl- secretion and intracellular Ca2+ levels ([Ca2+]i) in monolayers. PA potentiated the effect of carbachol on [Ca2+]i and Cl- secretion, although it did not stimulate Cl- secretion by itself. PA had divergent effects on cyclic nucleotide-dependent Cl- secretion. It delayed Cl- secretion induced by vasoactive intestinal polypeptide [VIP, adenosine 3',5'-cyclic monophosphate (cAMP) dependent] but potentiated that induced by the heat-stable enterotoxin of Escherichia coli (STa; guanosine 3',5'-cyclic monophosphate dependent). PA did not alter AMP or GMP levels, suggesting that PA acts at a site distal to the generation of these second messengers. PA caused a slight increase in phosphorylation of protein kinase C substrates but not of cAMP-dependent protein kinase substrates. However, PA is probably not acting through a classical protein kinase C pathway, because we have previously shown that phorbol esters inhibit carbachol's actions, and the protein kinase C inhibitor staurosporine failed to block the effect of PA on VIP- or STa-stimulated Cl- secretion. Thus PA differentially regulates stimulated Cl- secretion in T84 cells, depending on the nature of the agonist.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

May 1993

Volume

264

Issue

5 Pt 1

Start / End Page

C1210 / C1218

Location

United States

Related Subject Headings

  • Vasoactive Intestinal Peptide
  • Tumor Cells, Cultured
  • Phosphorylation
  • Phosphoproteins
  • Phospholipids
  • Phosphatidylinositols
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidic Acids
  • Kinetics
  • Inositol Phosphates
 

Citation

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Vajanaphanich, M., Kachintorn, U., Barrett, K. E., Cohn, J. A., Dharmsathaphorn, K., & Traynor-Kaplan, A. (1993). Phosphatidic acid modulates Cl- secretion in T84 cells: varying effects depending on mode of stimulation. Am J Physiol, 264(5 Pt 1), C1210–C1218. https://doi.org/10.1152/ajpcell.1993.264.5.C1210
Vajanaphanich, M., U. Kachintorn, K. E. Barrett, J. A. Cohn, K. Dharmsathaphorn, and A. Traynor-Kaplan. “Phosphatidic acid modulates Cl- secretion in T84 cells: varying effects depending on mode of stimulation.Am J Physiol 264, no. 5 Pt 1 (May 1993): C1210–18. https://doi.org/10.1152/ajpcell.1993.264.5.C1210.
Vajanaphanich M, Kachintorn U, Barrett KE, Cohn JA, Dharmsathaphorn K, Traynor-Kaplan A. Phosphatidic acid modulates Cl- secretion in T84 cells: varying effects depending on mode of stimulation. Am J Physiol. 1993 May;264(5 Pt 1):C1210–8.
Vajanaphanich, M., et al. “Phosphatidic acid modulates Cl- secretion in T84 cells: varying effects depending on mode of stimulation.Am J Physiol, vol. 264, no. 5 Pt 1, May 1993, pp. C1210–18. Pubmed, doi:10.1152/ajpcell.1993.264.5.C1210.
Vajanaphanich M, Kachintorn U, Barrett KE, Cohn JA, Dharmsathaphorn K, Traynor-Kaplan A. Phosphatidic acid modulates Cl- secretion in T84 cells: varying effects depending on mode of stimulation. Am J Physiol. 1993 May;264(5 Pt 1):C1210–C1218.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

May 1993

Volume

264

Issue

5 Pt 1

Start / End Page

C1210 / C1218

Location

United States

Related Subject Headings

  • Vasoactive Intestinal Peptide
  • Tumor Cells, Cultured
  • Phosphorylation
  • Phosphoproteins
  • Phospholipids
  • Phosphatidylinositols
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidic Acids
  • Kinetics
  • Inositol Phosphates