Submucosal glands are the predominant site of CFTR expression in the human bronchus.

Published

Journal Article

We have used in situ hybridization and immunocytochemistry to characterize the cellular distribution of cystic fibrosis (CF) gene expression in human bronchus. The cystic fibrosis transmembrane conductance regular (CFTR) was primarily localized to cells of submucosal glands in bronchial tissues from non-CF individuals notably in the serous component of the secretory tubules as well as a subpopulation of cells in ducts. Normal distribution of CFTR mRNA was found in CF tissues while expression of CFTR protein was genotype specific, with delta F508 homozygotes demonstrating no detectable protein and compound heterozygotes expressing decreased levels of normally distributed protein. Our data suggest mechanisms whereby defects in CFTR expression could lead to abnormal production of mucus in human lung.

Full Text

Duke Authors

Cited Authors

  • Engelhardt, JF; Yankaskas, JR; Ernst, SA; Yang, Y; Marino, CR; Boucher, RC; Cohn, JA; Wilson, JM

Published Date

  • November 1992

Published In

Volume / Issue

  • 2 / 3

Start / End Page

  • 240 - 248

PubMed ID

  • 1285365

Pubmed Central ID

  • 1285365

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng1192-240

Language

  • eng

Conference Location

  • United States