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K-ras mutations in Müllerian inclusion cysts associated with serous borderline tumors of the ovary.

Publication ,  Journal Article
Alvarez, AA; Moore, WF; Robboy, SJ; Bentley, RC; Gumbs, C; Futreal, PA; Berchuck, A
Published in: Gynecol Oncol
February 2001

OBJECTIVE: Müllerian inclusion cysts (MIC) are small benign appearing glands that are occasionally noted in lymph nodes and peritoneal biopsies. They occur most frequently in women with serous ovarian tumors, with borderline tumors (SBOT) having a higher incidence than invasive cancers. The aim of this study was to examine whether MIC and SBOT have identical K-ras mutations, which would suggest that they are related. Methods. Six patients in whom adequate tissue was available from SBOT, MIC, and normal tissue were identified from a consecutive series of patients with SBOT who underwent lymph node sampling from 1992 to 1997 at Duke University Medical Center. DNA extraction was performed using laser capture microdissection. Exon 1 of the K-ras gene was amplified using PCR and subjected to single-strand conformation analysis to screen for mutations. Shifted bands were sequenced to confirm the presence of mutations. RESULTS: Mutations in codon 12 of K-ras were found in three of six (50%) SBOT. In two of these three cases, the identical mutation was found in the SBOT and the MIC (gly to val in both cases), but not in the corresponding normal DNA. In one case, a mutation was seen in the ovarian tumor (gly to asp), but not in the corresponding MIC. CONCLUSIONS: Mutations in codon 12 of the K-ras gene are a hallmark of serous borderline tumors. The presence of identical K-ras mutations in some SBOT and their associated MIC suggests that they are related processes. Both may arise due to a field effect, or alternatively some MIC may represent metastases from the primary ovarian tumor.

Duke Scholars

Published In

Gynecol Oncol

DOI

ISSN

0090-8258

Publication Date

February 2001

Volume

80

Issue

2

Start / End Page

201 / 206

Location

United States

Related Subject Headings

  • Peritoneal Cavity
  • Ovarian Neoplasms
  • Oncology & Carcinogenesis
  • Mutation
  • Mullerian Ducts
  • Middle Aged
  • Lymph Nodes
  • Humans
  • Genes, ras
  • Female
 

Citation

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Alvarez, A. A., Moore, W. F., Robboy, S. J., Bentley, R. C., Gumbs, C., Futreal, P. A., & Berchuck, A. (2001). K-ras mutations in Müllerian inclusion cysts associated with serous borderline tumors of the ovary. Gynecol Oncol, 80(2), 201–206. https://doi.org/10.1006/gyno.2000.6066
Alvarez, A. A., W. F. Moore, S. J. Robboy, R. C. Bentley, C. Gumbs, P. A. Futreal, and A. Berchuck. “K-ras mutations in Müllerian inclusion cysts associated with serous borderline tumors of the ovary.Gynecol Oncol 80, no. 2 (February 2001): 201–6. https://doi.org/10.1006/gyno.2000.6066.
Alvarez AA, Moore WF, Robboy SJ, Bentley RC, Gumbs C, Futreal PA, et al. K-ras mutations in Müllerian inclusion cysts associated with serous borderline tumors of the ovary. Gynecol Oncol. 2001 Feb;80(2):201–6.
Alvarez, A. A., et al. “K-ras mutations in Müllerian inclusion cysts associated with serous borderline tumors of the ovary.Gynecol Oncol, vol. 80, no. 2, Feb. 2001, pp. 201–06. Pubmed, doi:10.1006/gyno.2000.6066.
Alvarez AA, Moore WF, Robboy SJ, Bentley RC, Gumbs C, Futreal PA, Berchuck A. K-ras mutations in Müllerian inclusion cysts associated with serous borderline tumors of the ovary. Gynecol Oncol. 2001 Feb;80(2):201–206.
Journal cover image

Published In

Gynecol Oncol

DOI

ISSN

0090-8258

Publication Date

February 2001

Volume

80

Issue

2

Start / End Page

201 / 206

Location

United States

Related Subject Headings

  • Peritoneal Cavity
  • Ovarian Neoplasms
  • Oncology & Carcinogenesis
  • Mutation
  • Mullerian Ducts
  • Middle Aged
  • Lymph Nodes
  • Humans
  • Genes, ras
  • Female