Sodium-independent binding of L-[3H]proline to hippocampal synaptic membranes.
Journal Article (Journal Article)
L-[3H]Proline binds specifically and saturably to rat hippocampal synaptic membranes in the absence of inorganic ions. Saturation isotherms constructed in Hepes-Tris buffer best fit a model of a single independent population of binding sites with a dissociation constant of 1.6 microM and maximum density of 16 pmol/mg protein. The rank order of displacement of radioligand by proline analogues suggests that binding sites of this type could be receptors responsible for proline-induced retinal spreading depression and inhibition of memory consolidation.
Full Text
Duke Authors
Cited Authors
- Greene, WM; Wang, A; Nadler, JV
Published Date
- November 4, 1986
Published In
Volume / Issue
- 130 / 3
Start / End Page
- 333 - 336
PubMed ID
- 3792455
International Standard Serial Number (ISSN)
- 0014-2999
Digital Object Identifier (DOI)
- 10.1016/0014-2999(86)90288-8
Language
- eng
Conference Location
- Netherlands