Sodium-independent binding of L-[3H]proline to hippocampal synaptic membranes.

Journal Article (Journal Article)

L-[3H]Proline binds specifically and saturably to rat hippocampal synaptic membranes in the absence of inorganic ions. Saturation isotherms constructed in Hepes-Tris buffer best fit a model of a single independent population of binding sites with a dissociation constant of 1.6 microM and maximum density of 16 pmol/mg protein. The rank order of displacement of radioligand by proline analogues suggests that binding sites of this type could be receptors responsible for proline-induced retinal spreading depression and inhibition of memory consolidation.

Full Text

Duke Authors

Cited Authors

  • Greene, WM; Wang, A; Nadler, JV

Published Date

  • November 4, 1986

Published In

Volume / Issue

  • 130 / 3

Start / End Page

  • 333 - 336

PubMed ID

  • 3792455

International Standard Serial Number (ISSN)

  • 0014-2999

Digital Object Identifier (DOI)

  • 10.1016/0014-2999(86)90288-8


  • eng

Conference Location

  • Netherlands