Loss of tumor suppressor gene expression in high-grade but not low-grade non-Hodgkin's lymphomas.
Published
Journal Article
The products of the MTS1/CDKN2 and retinoblastoma (RB) tumor suppressor genes, p16 and pRB, act as agonists in controlling the late G1 cell cycle checkpoint. Inactivation of either gene occurs in a wide range of human malignant neoplasms. Data on the expression of both genes in the same set of malignant lymphoid neoplasms are scarce. We studied the p16/pRB pathway in low-grade and high-grade non-Hodgkin's lymphomas, using immunohistochemical techniques. Paraffin sections of 9 reactive lymph nodes and 43 low-grade and 60 high-grade malignant lymphomas were reacted with antibodies against pRB and p16. All benign lymph nodes showed a normal pattern of RB and MTS1/CDKN2 expression. Of 101 evaluable lymphomas, only a single high-grade tumor displayed loss of RB reactivity. Loss of p16 was identified in 14 of 55 evaluable high-grade lymphomas but not in any of the low-grade lesions. All but 3 of the RB- and p16-negative cases were diffuse large cell lymphomas, for an abnormality rate of 55% in this category. While loss of RB function was a rare event in human lymphomagenesis, p16 was absent in some 25% of high-grade non-Hodgkin's lymphomas; diffuse large cell lymphomas were the primary target of tumor suppressor gene inactivation.
Full Text
Duke Authors
Cited Authors
- Geradts, J; Andriko, JW; Abbondanzo, SL
Published Date
- June 1998
Published In
Volume / Issue
- 109 / 6
Start / End Page
- 669 - 674
PubMed ID
- 9620022
Pubmed Central ID
- 9620022
International Standard Serial Number (ISSN)
- 0002-9173
Digital Object Identifier (DOI)
- 10.1093/ajcp/109.6.669
Language
- eng
Conference Location
- England