Retinoblastoma protein monoclonal antibodies with novel characteristics.

Journal Article

We have developed a family of monoclonal antibodies directed against the retinoblastoma gene product (p110RB). One of these monoclonal antibodies, 3C8, binds p110RB near the C-terminal end of the protein (aa886-aa905). It was characterized by immunoblotting, ELISA, fluorescence-activated flow cytometry and immunohistostaining. It was shown to be useful for the detection of p110RB in formalin-fixed and paraffin-embedded tissue sections. Because 3C8 binds outside of regions shown to be involved in p110RB interactions with other cellular proteins, it may be an especially useful reagent for the reliable detection of p110RB in tumor cells, and for the isolation by affinity chromatography of p110RB complexes with other cellular proteins.

Full Text

Duke Authors

Cited Authors

  • Wen, SF; Nodelman, M; Nared-Hood, K; Duncan, J; Geradts, J; Shepard, HM

Published Date

  • March 10, 1994

Published In

Volume / Issue

  • 169 / 2

Start / End Page

  • 231 - 240

PubMed ID

  • 7510760

International Standard Serial Number (ISSN)

  • 0022-1759

Language

  • eng

Conference Location

  • Netherlands