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Retinoblastoma protein monoclonal antibodies with novel characteristics.

Publication ,  Journal Article
Wen, SF; Nodelman, M; Nared-Hood, K; Duncan, J; Geradts, J; Shepard, HM
Published in: J Immunol Methods
March 10, 1994

We have developed a family of monoclonal antibodies directed against the retinoblastoma gene product (p110RB). One of these monoclonal antibodies, 3C8, binds p110RB near the C-terminal end of the protein (aa886-aa905). It was characterized by immunoblotting, ELISA, fluorescence-activated flow cytometry and immunohistostaining. It was shown to be useful for the detection of p110RB in formalin-fixed and paraffin-embedded tissue sections. Because 3C8 binds outside of regions shown to be involved in p110RB interactions with other cellular proteins, it may be an especially useful reagent for the reliable detection of p110RB in tumor cells, and for the isolation by affinity chromatography of p110RB complexes with other cellular proteins.

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Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

March 10, 1994

Volume

169

Issue

2

Start / End Page

231 / 240

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Retinoblastoma Protein
  • Retinoblastoma
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Immunoenzyme Techniques
  • Immunoblotting
  • Humans
  • Hela Cells
 

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Wen, S. F., Nodelman, M., Nared-Hood, K., Duncan, J., Geradts, J., & Shepard, H. M. (1994). Retinoblastoma protein monoclonal antibodies with novel characteristics. J Immunol Methods, 169(2), 231–240. https://doi.org/10.1016/0022-1759(94)90267-4
Wen, S. F., M. Nodelman, K. Nared-Hood, J. Duncan, J. Geradts, and H. M. Shepard. “Retinoblastoma protein monoclonal antibodies with novel characteristics.J Immunol Methods 169, no. 2 (March 10, 1994): 231–40. https://doi.org/10.1016/0022-1759(94)90267-4.
Wen SF, Nodelman M, Nared-Hood K, Duncan J, Geradts J, Shepard HM. Retinoblastoma protein monoclonal antibodies with novel characteristics. J Immunol Methods. 1994 Mar 10;169(2):231–40.
Wen, S. F., et al. “Retinoblastoma protein monoclonal antibodies with novel characteristics.J Immunol Methods, vol. 169, no. 2, Mar. 1994, pp. 231–40. Pubmed, doi:10.1016/0022-1759(94)90267-4.
Wen SF, Nodelman M, Nared-Hood K, Duncan J, Geradts J, Shepard HM. Retinoblastoma protein monoclonal antibodies with novel characteristics. J Immunol Methods. 1994 Mar 10;169(2):231–240.
Journal cover image

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

March 10, 1994

Volume

169

Issue

2

Start / End Page

231 / 240

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Retinoblastoma Protein
  • Retinoblastoma
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Immunoenzyme Techniques
  • Immunoblotting
  • Humans
  • Hela Cells