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Use of immunotoxins in combination to inhibit clonogenic growth of human breast carcinoma cells.

Publication ,  Journal Article
Yu, YH; Crews, JR; Cooper, K; Ramakrishnan, S; Houston, LL; Leslie, DS; George, SL; Lidor, Y; Boyer, CM; Ring, DB
Published in: Cancer Res
June 1, 1990

Substantial heterogeneity has been observed in the expression of individual antigens within tumor cell populations. Immunotoxins which bind to different cell surface antigens might exert additive or synergistic cytotoxicity when used in combination to eliminate all clonogenic cells within a tumor. Immunotoxins have been prepared by conjugating recombinantly derived toxin A chain to different monoclonal reagents which recognize cell surface determinants of Mr 42,000 (317G5), 55,000 (260F9), and 200,000 (741F8). Each immunotoxin was evaluated for binding, internalization, and cytotoxicity with four breast cancer cell lines. Each of the three immunotoxins bound to the SKBr3 cell line and exerted antitumor activity in a limiting dilution clonogenic assay. Simultaneous treatment with two immunotoxins produced additive antitumor activity with each of the possible combinations. Additive binding could be demonstrated by immunofluorescent techniques, however, with only one of three combinations. With two of the three combinations, subpopulations of tumor cells could be identified which lacked one or the other antigenic determinant but not both. Consequently, log-additive antitumor activity was produced by immunotoxins in combination, and heterogeneity of antigenic targets may have contributed to the combined cytotoxicity in some but not all cases.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

June 1, 1990

Volume

50

Issue

11

Start / End Page

3231 / 3238

Location

United States

Related Subject Headings

  • Tumor Stem Cell Assay
  • Tumor Cells, Cultured
  • Recombinant Proteins
  • Oncology & Carcinogenesis
  • Molecular Weight
  • Immunotoxins
  • Humans
  • Breast Neoplasms
  • Antigens, Neoplasm
  • Antibodies, Monoclonal
 

Citation

APA
Chicago
ICMJE
MLA
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Yu, Y. H., Crews, J. R., Cooper, K., Ramakrishnan, S., Houston, L. L., Leslie, D. S., … Ring, D. B. (1990). Use of immunotoxins in combination to inhibit clonogenic growth of human breast carcinoma cells. Cancer Res, 50(11), 3231–3238.
Yu, Y. H., J. R. Crews, K. Cooper, S. Ramakrishnan, L. L. Houston, D. S. Leslie, S. L. George, Y. Lidor, C. M. Boyer, and D. B. Ring. “Use of immunotoxins in combination to inhibit clonogenic growth of human breast carcinoma cells.Cancer Res 50, no. 11 (June 1, 1990): 3231–38.
Yu YH, Crews JR, Cooper K, Ramakrishnan S, Houston LL, Leslie DS, et al. Use of immunotoxins in combination to inhibit clonogenic growth of human breast carcinoma cells. Cancer Res. 1990 Jun 1;50(11):3231–8.
Yu, Y. H., et al. “Use of immunotoxins in combination to inhibit clonogenic growth of human breast carcinoma cells.Cancer Res, vol. 50, no. 11, June 1990, pp. 3231–38.
Yu YH, Crews JR, Cooper K, Ramakrishnan S, Houston LL, Leslie DS, George SL, Lidor Y, Boyer CM, Ring DB. Use of immunotoxins in combination to inhibit clonogenic growth of human breast carcinoma cells. Cancer Res. 1990 Jun 1;50(11):3231–3238.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

June 1, 1990

Volume

50

Issue

11

Start / End Page

3231 / 3238

Location

United States

Related Subject Headings

  • Tumor Stem Cell Assay
  • Tumor Cells, Cultured
  • Recombinant Proteins
  • Oncology & Carcinogenesis
  • Molecular Weight
  • Immunotoxins
  • Humans
  • Breast Neoplasms
  • Antigens, Neoplasm
  • Antibodies, Monoclonal