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Cloning efficiency of cultured human tumor cell lines measured with the use of a Coulter particle counter.

Publication ,  Journal Article
Hazelton, BJ; Torrance, PM; George, SL; Houghton, PJ
Published in: J Natl Cancer Inst
September 1984

A technique is described by which colony formation in agarose may be rapidly and reproducibly determined with the use of a modified Coulter particle counter (CPC). The cloning efficiency of RD human rhabdomyosarcoma cells after exposure to vincristine sulfate or cisplatin has been compared with the CPC method or by conventional visual counting. These techniques give very similar results. In combination with a Coulter Channelyzer, the CPC technique can be used to evaluate drug effects in colony sizes from a median of 20 to greater than 290 cells. The integrity of colonies formed by 3 colon carcinoma cell lines was examined to determine if this method could be applied to other systems.

Duke Scholars

Published In

J Natl Cancer Inst

ISSN

0027-8874

Publication Date

September 1984

Volume

73

Issue

3

Start / End Page

555 / 563

Location

United States

Related Subject Headings

  • Vincristine
  • Rhabdomyosarcoma
  • Oncology & Carcinogenesis
  • Kinetics
  • Humans
  • Culture Techniques
  • Colonic Neoplasms
  • Clone Cells
  • Cisplatin
  • Cell Line
 

Citation

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Hazelton, B. J., Torrance, P. M., George, S. L., & Houghton, P. J. (1984). Cloning efficiency of cultured human tumor cell lines measured with the use of a Coulter particle counter. J Natl Cancer Inst, 73(3), 555–563.
Hazelton, B. J., P. M. Torrance, S. L. George, and P. J. Houghton. “Cloning efficiency of cultured human tumor cell lines measured with the use of a Coulter particle counter.J Natl Cancer Inst 73, no. 3 (September 1984): 555–63.
Hazelton BJ, Torrance PM, George SL, Houghton PJ. Cloning efficiency of cultured human tumor cell lines measured with the use of a Coulter particle counter. J Natl Cancer Inst. 1984 Sep;73(3):555–63.
Hazelton, B. J., et al. “Cloning efficiency of cultured human tumor cell lines measured with the use of a Coulter particle counter.J Natl Cancer Inst, vol. 73, no. 3, Sept. 1984, pp. 555–63.
Hazelton BJ, Torrance PM, George SL, Houghton PJ. Cloning efficiency of cultured human tumor cell lines measured with the use of a Coulter particle counter. J Natl Cancer Inst. 1984 Sep;73(3):555–563.
Journal cover image

Published In

J Natl Cancer Inst

ISSN

0027-8874

Publication Date

September 1984

Volume

73

Issue

3

Start / End Page

555 / 563

Location

United States

Related Subject Headings

  • Vincristine
  • Rhabdomyosarcoma
  • Oncology & Carcinogenesis
  • Kinetics
  • Humans
  • Culture Techniques
  • Colonic Neoplasms
  • Clone Cells
  • Cisplatin
  • Cell Line