A synthetic peptide homologous to retroviral envelope protein down-regulates TNF-alpha and IFN-gamma mRNA expression.
Journal Article
We investigated the influence of CKS-17, a synthetic heptadecapeptide that corresponds to a highly conserved domain of the immunosuppressive retroviral envelope protein p15E, on staphylococcal enterotoxin B (SEB)-induced TNF-alpha gene expression in human peripheral blood mononuclear cells and highly purified human monocyte preparations, as well as the production of TNF-alpha protein, using human peripheral blood mononuclear cells. RNA hybridization studies show that CKS-17 inhibits SEB-induced TNF-alpha mRNA accumulation in human peripheral blood mononuclear cells and human monocytes. CKS-17 is also shown to be highly suppressive for SEB-induced production of TNF-alpha proteins. Similarly, CKS-17 inhibits expression of SEB-induced IFN-gamma mRNA in human peripheral blood mononuclear cells. These results suggest that CKS-17 down-regulates both TNF-alpha and IFN-gamma production at mRNA level.
Full Text
Duke Authors
Cited Authors
- Haraguchi, S; Good, RA; Cianciolo, GJ; Day, NK
Published Date
- October 1992
Published In
Volume / Issue
- 52 / 4
Start / End Page
- 469 - 472
PubMed ID
- 1402393
Pubmed Central ID
- 1402393
International Standard Serial Number (ISSN)
- 0741-5400
Digital Object Identifier (DOI)
- 10.1002/jlb.52.4.469
Language
- eng
Conference Location
- United States