The rapid and large scale separation of peritoneal macrophages.

Journal Article (Journal Article)

A simple method for rapid and large scale (over 109 cells) separation of peritoneal macrophages by incubation in a glass-bead column and elution with buffered ethylenediamine tetraacetic acid (EDTA) solution is described. Macrophages obtained were about 90 per cent pure with the recovery rates of 38–53 per cent depending upon the sample size, and 83 per cent viability. Lymphocyte contamination was reduced to 5 per cent or less.

Full Text

Duke Authors

Cited Authors

  • Shaipanich, T; Cianciolo, GJ

Published Date

  • April 1, 1972

Published In

Volume / Issue

  • 22 / 4

Start / End Page

  • 525 - 529

PubMed ID

  • 4622703

Pubmed Central ID

  • PMC1407822

International Standard Serial Number (ISSN)

  • 0019-2805

Language

  • eng

Conference Location

  • England