Mapping of antibody specificities to VH gene families.

Published

Journal Article

VH gene segments represent the products of the repeated duplication and subsequent diversification of a primordial V gene element. It is widely assumed that natural selection, operating via pathogens, has played the dominant role in this process. Here, we screen some 3.7 x 10(4) C mu+ colonies of mitogen-activated B cells for the production of antibodies specific for phosphorylcholine or hen egg lysozyme and expression of the VH X-24, S107, Q52, or J558 gene families. These gene families were expressed at frequencies proportional to their genomic complexity among both unselected and antigen-specific C mu+ colonies. Thus, the capacity to encode equivalent antibody-combining sites is dispersed uniformly among VH families. This result suggests that individual VH genes have not evolved to address specific antigens.

Full Text

Duke Authors

Cited Authors

  • Kelsoe, G; Miceli, R; Cerny, J; Schulze, DH

Published Date

  • 1989

Published In

Volume / Issue

  • 29 / 5

Start / End Page

  • 288 - 296

PubMed ID

  • 2785504

Pubmed Central ID

  • 2785504

International Standard Serial Number (ISSN)

  • 0093-7711

Digital Object Identifier (DOI)

  • 10.1007/bf00352838

Language

  • eng

Conference Location

  • United States