Contribution of the VH11 gene family to mitogen-responsive B cell repertoire in C57BL/6 mice.

Journal Article

The contribution of VH11 gene family to the development of the primary B cell repertoire has been studied by analyzing 1.8 x 10(4) mitogen induced B lymphocyte colonies. The data demonstrate that VH11 family is predominantly expressed among neonatal splenic as well as adult peritoneal B cell colonies, both rich in Ly-1+ B cells. VH11 gene family expression among B splenocytes decreases during ontogeny and VH11 family pairs stochastically with different V kappa families among mitogen-activated neonatal B cell colonies, which are representative of an antigen unselected B cell repertoire. Thus, an increased VH11 expression among peritoneal and neonatal B cells points towards its biased expression among Ly-1+ B lymphocytes. The restricted V gene rearrangements and VH11-V kappa 9 pairing observed among anti-bromelain-treated mouse red blood cells autoantibodies are likely to be an outcome of both intrinsic gene recombination processes per se as well as selection by an autoantigen and/or local selective environmental factors.

Full Text

Duke Authors

Cited Authors

  • Kaushik, A; Reininger, L; Kelsoe, G; Jaton, JC; Bona, C

Published Date

  • March 1, 1991

Published In

Volume / Issue

  • 21 / 3

Start / End Page

  • 827 - 830

PubMed ID

  • 1901267

International Standard Serial Number (ISSN)

  • 0014-2980

Digital Object Identifier (DOI)

  • 10.1002/eji.1830210344

Language

  • eng

Conference Location

  • Germany