RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues.
Journal Article (Journal Article)
We generated mice in which a functional RAG2:GFP fusion gene is knocked in to the endogenous RAG2 locus. In bone marrow and thymus, RAG2:GFP expression occurs in appropriate stages of developing B and T cells as well as in immature bone marrow IgM+ B cells. RAG2:GFP also is expressed in IgD+ B cells following cross-linking of IgM on immature IgM+ IgD+ B cells generated in vitro. RAG2:GFP expression is undetectable in most immature splenic B cells; however, in young RAG2:GFP mice, there are substantial numbers of splenic RAG2:GFP+ cells that mostly resemble pre-B cells. The latter population decreases in size with age but reappears following immunization of older RAG2:GFP mice. We discuss the implications of these findings for current models of receptor assembly and diversification.
Full Text
Duke Authors
Cited Authors
- Monroe, RJ; Seidl, KJ; Gaertner, F; Han, S; Chen, F; Sekiguchi, J; Wang, J; Ferrini, R; Davidson, L; Kelsoe, G; Alt, FW
Published Date
- August 1999
Published In
Volume / Issue
- 11 / 2
Start / End Page
- 201 - 212
PubMed ID
- 10485655
International Standard Serial Number (ISSN)
- 1074-7613
Digital Object Identifier (DOI)
- 10.1016/s1074-7613(00)80095-3
Language
- eng
Conference Location
- United States