Transcriptome analysis of mouse stem cells and early embryos.

Published

Journal Article

Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that include oocytes, blastocysts, and embryonic and adult stem cells, we obtained 249,200 high-quality EST sequences and clustered them with public sequences to produce an index of approximately 30,000 total mouse genes that includes 977 previously unidentified genes. Analysis of gene expression levels by EST frequency identifies genes that characterize preimplantation embryos, embryonic stem cells, and adult stem cells, thus providing potential markers as well as clues to the functional features of these cells. Principal component analysis identified a set of 88 genes whose average expression levels decrease from oocytes to blastocysts, stem cells, postimplantation embryos, and finally to newborn tissues. This can be a first step towards a possible definition of a molecular scale of cellular potency. The sequences and cDNA clones recovered in this work provide a comprehensive resource for genes functioning in early mouse embryos and stem cells. The nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine.

Full Text

Duke Authors

Cited Authors

  • Sharov, AA; Piao, Y; Matoba, R; Dudekula, DB; Qian, Y; VanBuren, V; Falco, G; Martin, PR; Stagg, CA; Bassey, UC; Wang, Y; Carter, MG; Hamatani, T; Aiba, K; Akutsu, H; Sharova, L; Tanaka, TS; Kimber, WL; Yoshikawa, T; Jaradat, SA; Pantano, S; Nagaraja, R; Boheler, KR; Taub, D; Hodes, RJ; Longo, DL; Schlessinger, D; Keller, J; Klotz, E; Kelsoe, G; Umezawa, A; Vescovi, AL; Rossant, J; Kunath, T; Hogan, BLM; Curci, A; D'Urso, M; Kelso, J; Hide, W; Ko, MSH

Published Date

  • December 2003

Published In

Volume / Issue

  • 1 / 3

Start / End Page

  • E74 -

PubMed ID

  • 14691545

Pubmed Central ID

  • 14691545

Electronic International Standard Serial Number (EISSN)

  • 1545-7885

Digital Object Identifier (DOI)

  • 10.1371/journal.pbio.0000074

Language

  • eng

Conference Location

  • United States