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Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.

Publication ,  Journal Article
Han, S; Hathcock, K; Zheng, B; Kepler, TB; Hodes, R; Kelsoe, G
Published in: J Immunol
July 15, 1995

Costimulatory interactions between T and B lymphocytes are crucial for T cell activation and B cell proliferation and differentiation. We have compared the roles of CD40L and B7-2 in the initiation and maturation of humoral immunity by administering anti-CD40 ligand (L) or anti-B7-2 Ab during the early (days -1 to 3) or late (days 6-10) phases of primary responses to thymus-dependent (Td) and -independent (Ti) Ags. Germinal center (GC) formation in response to a Td Ag was inhibited completely by the early administration of anti-CD40L or anti-B7-2 Abs. Later in the response, established GCs remained sensitive to anti-CD40L but were resistant to treatment with anti-B7-2. However, Ig hypermutation was reduced dramatically in GCs of anti-B7-2-treated mice and humoral memory was impaired. Early administration of anti-CD40L reduced serum Ab levels to approximately 10% of controls, whereas early treatment with anti-B7-2 reduced Ab production by only 50%. Later treatments with either Ab had no effect on Ab production. Response to a type II Ti Ag was more resistant than Td responses to interruption of costimulatory interactions. Our findings suggest that the costimulatory roles of CD40:CD40L and B7-2:CD28/CTLA-4 differ in the GC; administration of anti-CD40L abrogates an established GC reaction, whereas Ab to B7-2 suppresses Ig hypermutation and entry into the B cell memory compartment. Once B cells have entered the differentiation pathway to Ab production, neither CD40L nor B7-2 is necessary for their continued differentiation and persistence.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1995

Volume

155

Issue

2

Start / End Page

556 / 567

Location

United States

Related Subject Headings

  • Streptococcus pneumoniae
  • Mutation
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice
  • Membrane Glycoproteins
  • Lymphocyte Activation
  • Lymph Nodes
  • Ligands
  • Immunology
 

Citation

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Han, S., Hathcock, K., Zheng, B., Kepler, T. B., Hodes, R., & Kelsoe, G. (1995). Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers. J Immunol, 155(2), 556–567.
Han, S., K. Hathcock, B. Zheng, T. B. Kepler, R. Hodes, and G. Kelsoe. “Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.J Immunol 155, no. 2 (July 15, 1995): 556–67.
Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G. Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers. J Immunol. 1995 Jul 15;155(2):556–67.
Han, S., et al. “Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.J Immunol, vol. 155, no. 2, July 1995, pp. 556–67.
Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G. Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers. J Immunol. 1995 Jul 15;155(2):556–567.

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1995

Volume

155

Issue

2

Start / End Page

556 / 567

Location

United States

Related Subject Headings

  • Streptococcus pneumoniae
  • Mutation
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice
  • Membrane Glycoproteins
  • Lymphocyte Activation
  • Lymph Nodes
  • Ligands
  • Immunology