Immunodiagnosis of infection with Schistosoma mansoni: enzyme-linked immunosorbent assay for detection of antibody to circulating antigen.

Published

Journal Article

A circulating antigen, a negatively charged polysaccharide from the trematode Schistosoma mansoni, was noncovalently bound to the surface of poly(L-lysine)-coated wells in polystyrene trays, which were then used in a micro-enzyme-linked immunosorbent assay (ELISA) test. The method provides an immunodiagnostic test for schistosomiasis of exceptional sensitivity with a high degree of specificity. Comparison of Bell egg counts and ELISA titers revealed a good correlation (r congruent to 0.80) in young individuals with low to moderate worm burdens, but this relationship was less marked in older individuals or those with high egg counts.

Full Text

Duke Authors

Cited Authors

  • Kelsoe, GH; Weller, TH

Published Date

  • November 1, 1978

Published In

Volume / Issue

  • 75 / 11

Start / End Page

  • 5715 - 5717

PubMed ID

  • 364488

Pubmed Central ID

  • 364488

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.75.11.5715

Language

  • eng

Conference Location

  • United States