Pairing of VH gene families with the lambda 1 light chain: evidence for a non-stochastic association.
Journal Article (Journal Article)
The frequencies at which four VH gene families pair with the lambda 1 light (L) chain were determined by sequential hybridization of VH- and lambda 1-specific DNA probes to mitogen-induced colonies of B cells. Analysis of pair frequencies indicates that the repertoire of lambda L chain antibodies is generated by the stochastic pairing of smaller 3'-to-mid-locus VH gene families (X-24, S107, Q52). However, the large 5' VH J558 family appeared to associate with the lambda 1 L chain non-stochastically; the frequency of VHJ558/lambda 1+ colonies among all lambda 1+ colonies was significantly lower than the frequency of J558 expression among all (C mu+) B cell colonies. This difference suggests that selection, either intrinsic at the level of rearrangement or heavy and L chain pairing, or extrinsic following surface immunoglobulin expression, may operate to shape the lambda antibody repertoire prior to the introduction of exogenous antigen.
Full Text
Duke Authors
Cited Authors
- Yurovsky, VV; Kelsoe, G
Published Date
- August 1993
Published In
Volume / Issue
- 23 / 8
Start / End Page
- 1975 - 1979
PubMed ID
- 8344362
International Standard Serial Number (ISSN)
- 0014-2980
Digital Object Identifier (DOI)
- 10.1002/eji.1830230837
Language
- eng
Conference Location
- Germany