Pairing of VH gene families with the lambda 1 light chain: evidence for a non-stochastic association.

Published

Journal Article

The frequencies at which four VH gene families pair with the lambda 1 light (L) chain were determined by sequential hybridization of VH- and lambda 1-specific DNA probes to mitogen-induced colonies of B cells. Analysis of pair frequencies indicates that the repertoire of lambda L chain antibodies is generated by the stochastic pairing of smaller 3'-to-mid-locus VH gene families (X-24, S107, Q52). However, the large 5' VH J558 family appeared to associate with the lambda 1 L chain non-stochastically; the frequency of VHJ558/lambda 1+ colonies among all lambda 1+ colonies was significantly lower than the frequency of J558 expression among all (C mu+) B cell colonies. This difference suggests that selection, either intrinsic at the level of rearrangement or heavy and L chain pairing, or extrinsic following surface immunoglobulin expression, may operate to shape the lambda antibody repertoire prior to the introduction of exogenous antigen.

Full Text

Duke Authors

Cited Authors

  • Yurovsky, VV; Kelsoe, G

Published Date

  • August 1993

Published In

Volume / Issue

  • 23 / 8

Start / End Page

  • 1975 - 1979

PubMed ID

  • 8344362

Pubmed Central ID

  • 8344362

International Standard Serial Number (ISSN)

  • 0014-2980

Digital Object Identifier (DOI)

  • 10.1002/eji.1830230837

Language

  • eng

Conference Location

  • Germany