The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development.

Published

Journal Article

Previous in vitro studies defined the minimal regions of RAG1 and RAG2 essential for V(D)J recombination. In order to characterize the role of the C-terminal "dispensable" portion of RAG2, we generated core-RAG2 knock-in mice. We found that the core-RAG2-containing recombinase complex is selectively defective in catalyzing V-to-DJ rearrangement at the IgH and TCRbeta loci, resulting in partial developmental blocks in B and T lymphopoiesis. Analysis of recombination intermediates showed defects at the cleavage phase of the reaction. We also observed a reduction in overall recombinase activity in core-RAG2-expressing thymocytes, leading us to suggest that the interaction of a defective recombinase with RSS sequences unique to VH and Vbeta gene segments may underlie the specific V-to-DJ rearrangement defect in core-RAG2 mice.

Full Text

Duke Authors

Cited Authors

  • Liang, H-E; Hsu, L-Y; Cado, D; Cowell, LG; Kelsoe, G; Schlissel, MS

Published Date

  • November 2002

Published In

Volume / Issue

  • 17 / 5

Start / End Page

  • 639 - 651

PubMed ID

  • 12433370

Pubmed Central ID

  • 12433370

International Standard Serial Number (ISSN)

  • 1074-7613

Language

  • eng

Conference Location

  • United States