A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development.
Journal Article (Journal Article)
To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.
- Hanzlik, AJ; Osemlak-Hanzlik, MM; Hauser, MA; Kurnit, DM
- January 1, 1993
Volume / Issue
- 3 / 1
Start / End Page
- 44 - 48
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States