A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development.

Journal Article

To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.

Full Text

Duke Authors

Cited Authors

  • Hanzlik, AJ; Osemlak-Hanzlik, MM; Hauser, MA; Kurnit, DM

Published Date

  • January 1993

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 44 - 48

PubMed ID

  • 8490653

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng0193-44

Language

  • eng

Conference Location

  • United States