Site-specific integration of the Haemophilus influenzae bacteriophage HP1. Identification of the points of recombinational strand exchange and the limits of the host attachment site.

Published

Journal Article

Isotopic transfer experiments and boundary replacement studies were used to define the size and cleavage points of the Haemophilus influenzae attB site for phage HP1 integration. The points of strand cleavage and transfer were separated by 5' extensions with a spacing or overlap region most probably 7 residues long. The complete HP1 attB site is included within an 18-base pair (bp) sequence surrounding the cleavage sites. The sequence of HP1 attB is remarkably symmetric. Two 8-bp inverted repeats surround the central residue of the 7-bp overlap sequence; this central residue is the second residue of the anticodon sequence of the H. influenzae tRNA(leu)(UUR) gene which contains attB, and this symmetric segment encodes the anticodon stem and loop.

Full Text

Duke Authors

Cited Authors

  • Hauser, MA; Scocca, JJ

Published Date

  • April 5, 1992

Published In

Volume / Issue

  • 267 / 10

Start / End Page

  • 6859 - 6864

PubMed ID

  • 1551893

Pubmed Central ID

  • 1551893

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States