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Site-specific integration of the Haemophilus influenzae bacteriophage HP1. Identification of the points of recombinational strand exchange and the limits of the host attachment site.

Publication ,  Journal Article
Hauser, MA; Scocca, JJ
Published in: J Biol Chem
April 5, 1992

Isotopic transfer experiments and boundary replacement studies were used to define the size and cleavage points of the Haemophilus influenzae attB site for phage HP1 integration. The points of strand cleavage and transfer were separated by 5' extensions with a spacing or overlap region most probably 7 residues long. The complete HP1 attB site is included within an 18-base pair (bp) sequence surrounding the cleavage sites. The sequence of HP1 attB is remarkably symmetric. Two 8-bp inverted repeats surround the central residue of the 7-bp overlap sequence; this central residue is the second residue of the anticodon sequence of the H. influenzae tRNA(leu)(UUR) gene which contains attB, and this symmetric segment encodes the anticodon stem and loop.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 5, 1992

Volume

267

Issue

10

Start / End Page

6859 / 6864

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • RNA, Transfer, Leu
  • Plasmids
  • Mutation
  • Molecular Sequence Data
  • Lysogeny
  • Haemophilus influenzae
  • Genes, Viral
  • DNA, Viral
  • Biochemistry & Molecular Biology
 

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 5, 1992

Volume

267

Issue

10

Start / End Page

6859 / 6864

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • RNA, Transfer, Leu
  • Plasmids
  • Mutation
  • Molecular Sequence Data
  • Lysogeny
  • Haemophilus influenzae
  • Genes, Viral
  • DNA, Viral
  • Biochemistry & Molecular Biology