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Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity.

Publication ,  Journal Article
Gulley, ML; Eagan, PA; Quintanilla-Martinez, L; Picado, AL; Smir, BN; Childs, C; Dunn, CD; Craig, FE; Williams, JW; Banks, PM
Published in: Blood
March 15, 1994

One hundred twenty-five cases of Hodgkin's disease from the United States (79), Mexico City (31), and Costa Rica (15) were analyzed for the presence of Epstein-Barr virus (EBV) by in situ hybridization to EBER1 transcripts. EBV was more frequently detected in the Reed-Sternberg (RS) cells of mixed cellularity Hodgkin's disease (37 of 48 [77%]) compared with the nodular sclerosis subtype (19 of 71 [27%], P < .001). The presence of EBV was also associated with Hispanic ethnicity (P < .001). In a multivariate analysis, patient age, gender, and geographic location were less predictive of EBV positivity than were mixed cellularity histology (odds ratio = 8.3) and Hispanic ethnicity (odds ratio = 4.3). Southern blot analysis of EBV terminal repeat fragments using the Xho1a probe showed that the viral DNA was monoclonal in 17 of 17 cases having EBER1-positive RS cells. By comparison, EBV DNA was not detected by Southern analysis in 20 cases lacking EBER1 in RS cells, even when occasional background lymphocytes expressed EBER1. Because clonal viral DNA was so readily detected in EBER1-positive cases, the EBV genome is probably amplified at least 50-fold in the infected RS cells. Monoclonality of EBV DNA implies that the RS cells were infected before malignant transformation.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

March 15, 1994

Volume

83

Issue

6

Start / End Page

1595 / 1602

Location

United States

Related Subject Headings

  • Reed-Sternberg Cells
  • Multivariate Analysis
  • Middle Aged
  • In Situ Hybridization
  • Immunology
  • Humans
  • Hodgkin Disease
  • Hispanic or Latino
  • Herpesvirus 4, Human
  • DNA, Viral
 

Citation

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MLA
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Gulley, M. L., Eagan, P. A., Quintanilla-Martinez, L., Picado, A. L., Smir, B. N., Childs, C., … Banks, P. M. (1994). Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity. Blood, 83(6), 1595–1602.
Gulley, M. L., P. A. Eagan, L. Quintanilla-Martinez, A. L. Picado, B. N. Smir, C. Childs, C. D. Dunn, F. E. Craig, J. W. Williams, and P. M. Banks. “Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity.Blood 83, no. 6 (March 15, 1994): 1595–1602.
Gulley ML, Eagan PA, Quintanilla-Martinez L, Picado AL, Smir BN, Childs C, et al. Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity. Blood. 1994 Mar 15;83(6):1595–602.
Gulley ML, Eagan PA, Quintanilla-Martinez L, Picado AL, Smir BN, Childs C, Dunn CD, Craig FE, Williams JW, Banks PM. Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity. Blood. 1994 Mar 15;83(6):1595–1602.

Published In

Blood

ISSN

0006-4971

Publication Date

March 15, 1994

Volume

83

Issue

6

Start / End Page

1595 / 1602

Location

United States

Related Subject Headings

  • Reed-Sternberg Cells
  • Multivariate Analysis
  • Middle Aged
  • In Situ Hybridization
  • Immunology
  • Humans
  • Hodgkin Disease
  • Hispanic or Latino
  • Herpesvirus 4, Human
  • DNA, Viral