Isolation and analysis of novel mutants of Escherichia coli prlA (secY).


Journal Article

Plasmid libraries of prlA mutants containing single-base-pair changes throughout the gene were generated by in vitro random mutagenesis. The prlA mutations capable of suppressing the secretion defect of LamB caused by mutations in the LamB signal peptide were selected and analyzed. Together with additional mutations generated by site-directed mutagenesis, a number of novel prlA mutations and/or suppressors were identified. These mutations provide the starting points for studying the relationship of structure and function of PrlA in its interaction with LamB and/or other component(s) in the Escherichia coli protein secretion-translocation complex.

Full Text

Cited Authors

  • Olsen, MK; Rosey, EL; Tomich, CS

Published Date

  • November 1993

Published In

Volume / Issue

  • 175 / 21

Start / End Page

  • 7092 - 7096

PubMed ID

  • 8226651

Pubmed Central ID

  • 8226651

Electronic International Standard Serial Number (EISSN)

  • 1098-5530

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.175.21.7092-7096.1993


  • eng