A simple Escherichia coli system for monitoring HIV protease activity: analysis of two temperature-sensitive protease mutants.

Published

Journal Article

A simple Escherichia coli system has been developed for the detection of human immunodeficiency virus (HIV) protease activity. In this system, the protease sequence is placed downstream of the HIV gag polypeptide in an operon arrangement. Upon expression of the operon, gag serves as the substrate for the protease; the level of protease activity can be determined by measurement of the cleavage product of gag in cell extracts by Western immunoblotting. This system is useful in both detection of protease mutations generated by mutagenesis and in testing substrate specificity of the protease by mutagenesis of the gag sequence. Using this system, we have observed that modification of the N-terminus of HIV protease renders the enzyme temperature sensitive; the temperature sensitivity is made more pronounced by the conserved change of valine to isoleucine at residue eleven.

Full Text

Cited Authors

  • Rockenbach, SK; Olsen, MK; Tomich, CS

Published Date

  • April 1990

Published In

Volume / Issue

  • 6 / 4

Start / End Page

  • 543 - 552

PubMed ID

  • 2187504

Pubmed Central ID

  • 2187504

Electronic International Standard Serial Number (EISSN)

  • 1931-8405

International Standard Serial Number (ISSN)

  • 0889-2229

Digital Object Identifier (DOI)

  • 10.1089/aid.1990.6.543

Language

  • eng