p53 does not repress hypoxia-induced transcription of the vascular endothelial growth factor gene.

Journal Article (Journal Article)

Hypoxia-induced neovascularization mediated by vascular endothelial growth factor (VEGF) contributes to tumor progression. Based on its effects when overexpressed in transient transfection assays, p53 has been proposed to repress VEGF transcription. To investigate this hypothesis, we have analyzed endogenous VEGF mRNA levels in Hep3B cells stably expressing an inducible p53-estrogen receptor fusion protein and in irradiated RKO cells expressing endogenous wild-type p53. In both cell lines, VEGF mRNA levels increased in response to hypoxia, either in the presence or absence of functional p53. Our data provide no evidence for a causal relationship between the loss of p53 activity and increased VEGF expression that is observed during tumor progression. Studies that attribute repressor functions to p53 based on analysis of cells transiently overexpressing this protein should be interpreted cautiously.

Full Text

Duke Authors

Cited Authors

  • Agani, F; Kirsch, DG; Friedman, SL; Kastan, MB; Semenza, GL

Published Date

  • October 15, 1997

Published In

Volume / Issue

  • 57 / 20

Start / End Page

  • 4474 - 4477

PubMed ID

  • 9377555

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng

Conference Location

  • United States