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p53 does not repress hypoxia-induced transcription of the vascular endothelial growth factor gene.

Publication ,  Journal Article
Agani, F; Kirsch, DG; Friedman, SL; Kastan, MB; Semenza, GL
Published in: Cancer Res
October 15, 1997

Hypoxia-induced neovascularization mediated by vascular endothelial growth factor (VEGF) contributes to tumor progression. Based on its effects when overexpressed in transient transfection assays, p53 has been proposed to repress VEGF transcription. To investigate this hypothesis, we have analyzed endogenous VEGF mRNA levels in Hep3B cells stably expressing an inducible p53-estrogen receptor fusion protein and in irradiated RKO cells expressing endogenous wild-type p53. In both cell lines, VEGF mRNA levels increased in response to hypoxia, either in the presence or absence of functional p53. Our data provide no evidence for a causal relationship between the loss of p53 activity and increased VEGF expression that is observed during tumor progression. Studies that attribute repressor functions to p53 based on analysis of cells transiently overexpressing this protein should be interpreted cautiously.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

October 15, 1997

Volume

57

Issue

20

Start / End Page

4474 / 4477

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Transfection
  • Transcription, Genetic
  • Recombinant Fusion Proteins
  • Receptors, Estrogen
  • RNA, Messenger
  • Oncology & Carcinogenesis
 

Citation

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Chicago
ICMJE
MLA
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Agani, F., Kirsch, D. G., Friedman, S. L., Kastan, M. B., & Semenza, G. L. (1997). p53 does not repress hypoxia-induced transcription of the vascular endothelial growth factor gene. Cancer Res, 57(20), 4474–4477.
Agani, F., D. G. Kirsch, S. L. Friedman, M. B. Kastan, and G. L. Semenza. “p53 does not repress hypoxia-induced transcription of the vascular endothelial growth factor gene.Cancer Res 57, no. 20 (October 15, 1997): 4474–77.
Agani F, Kirsch DG, Friedman SL, Kastan MB, Semenza GL. p53 does not repress hypoxia-induced transcription of the vascular endothelial growth factor gene. Cancer Res. 1997 Oct 15;57(20):4474–7.
Agani, F., et al. “p53 does not repress hypoxia-induced transcription of the vascular endothelial growth factor gene.Cancer Res, vol. 57, no. 20, Oct. 1997, pp. 4474–77.
Agani F, Kirsch DG, Friedman SL, Kastan MB, Semenza GL. p53 does not repress hypoxia-induced transcription of the vascular endothelial growth factor gene. Cancer Res. 1997 Oct 15;57(20):4474–4477.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

October 15, 1997

Volume

57

Issue

20

Start / End Page

4474 / 4477

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tumor Suppressor Protein p53
  • Tumor Cells, Cultured
  • Transfection
  • Transcription, Genetic
  • Recombinant Fusion Proteins
  • Receptors, Estrogen
  • RNA, Messenger
  • Oncology & Carcinogenesis