Use of gene expression profiling to direct in vivo molecular imaging of lung cancer.

Journal Article (Journal Article)

Using gene expression profiling, we identified cathepsin cysteine proteases as highly up-regulated genes in a mouse model of human lung adenocarcinoma. Overexpression of cathepsin proteases in these lung tumors was confirmed by immunohistochemistry and Western blotting. Therefore, an optical probe activated by cathepsin proteases was selected to detect murine lung tumors in vivo as small as 1 mm in diameter and spatially separated. We generated 3D maps of the fluorescence signal and fused them with anatomical computed tomography images to show a close correlation between fluorescence signal and tumor burden. By serially imaging the same mouse, optical imaging was used to follow tumor progression. This study demonstrates the capability for molecular imaging of a primary lung tumor by using endogenous proteases expressed by a tumor. It also highlights the feasibility of using gene expression profiling to identify molecular targets for imaging lung cancer.

Full Text

Duke Authors

Cited Authors

  • Grimm, J; Kirsch, DG; Windsor, SD; Kim, CFB; Santiago, PM; Ntziachristos, V; Jacks, T; Weissleder, R

Published Date

  • October 4, 2005

Published In

Volume / Issue

  • 102 / 40

Start / End Page

  • 14404 - 14409

PubMed ID

  • 16183744

Pubmed Central ID

  • PMC1242291

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0503920102


  • eng

Conference Location

  • United States