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Use of gene expression profiling to direct in vivo molecular imaging of lung cancer.

Publication ,  Journal Article
Grimm, J; Kirsch, DG; Windsor, SD; Kim, CFB; Santiago, PM; Ntziachristos, V; Jacks, T; Weissleder, R
Published in: Proc Natl Acad Sci U S A
October 4, 2005

Using gene expression profiling, we identified cathepsin cysteine proteases as highly up-regulated genes in a mouse model of human lung adenocarcinoma. Overexpression of cathepsin proteases in these lung tumors was confirmed by immunohistochemistry and Western blotting. Therefore, an optical probe activated by cathepsin proteases was selected to detect murine lung tumors in vivo as small as 1 mm in diameter and spatially separated. We generated 3D maps of the fluorescence signal and fused them with anatomical computed tomography images to show a close correlation between fluorescence signal and tumor burden. By serially imaging the same mouse, optical imaging was used to follow tumor progression. This study demonstrates the capability for molecular imaging of a primary lung tumor by using endogenous proteases expressed by a tumor. It also highlights the feasibility of using gene expression profiling to identify molecular targets for imaging lung cancer.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 4, 2005

Volume

102

Issue

40

Start / End Page

14404 / 14409

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tomography, Optical
  • Mice, Mutant Strains
  • Mice
  • Lung Neoplasms
  • Immunohistochemistry
  • Gene Expression Profiling
  • Fluorescence
  • Diagnostic Imaging
  • Cathepsins
 

Citation

APA
Chicago
ICMJE
MLA
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Grimm, J., Kirsch, D. G., Windsor, S. D., Kim, C. F. B., Santiago, P. M., Ntziachristos, V., … Weissleder, R. (2005). Use of gene expression profiling to direct in vivo molecular imaging of lung cancer. Proc Natl Acad Sci U S A, 102(40), 14404–14409. https://doi.org/10.1073/pnas.0503920102
Grimm, Jan, David G. Kirsch, Stephen D. Windsor, Carla F Bender Kim, Philip M. Santiago, Vasilis Ntziachristos, Tyler Jacks, and Ralph Weissleder. “Use of gene expression profiling to direct in vivo molecular imaging of lung cancer.Proc Natl Acad Sci U S A 102, no. 40 (October 4, 2005): 14404–9. https://doi.org/10.1073/pnas.0503920102.
Grimm J, Kirsch DG, Windsor SD, Kim CFB, Santiago PM, Ntziachristos V, et al. Use of gene expression profiling to direct in vivo molecular imaging of lung cancer. Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14404–9.
Grimm, Jan, et al. “Use of gene expression profiling to direct in vivo molecular imaging of lung cancer.Proc Natl Acad Sci U S A, vol. 102, no. 40, Oct. 2005, pp. 14404–09. Pubmed, doi:10.1073/pnas.0503920102.
Grimm J, Kirsch DG, Windsor SD, Kim CFB, Santiago PM, Ntziachristos V, Jacks T, Weissleder R. Use of gene expression profiling to direct in vivo molecular imaging of lung cancer. Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14404–14409.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 4, 2005

Volume

102

Issue

40

Start / End Page

14404 / 14409

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tomography, Optical
  • Mice, Mutant Strains
  • Mice
  • Lung Neoplasms
  • Immunohistochemistry
  • Gene Expression Profiling
  • Fluorescence
  • Diagnostic Imaging
  • Cathepsins