Telomere shortening in cultured autografts of patients with burns.

Journal Article (Journal Article)

In extensive third-degree burns, donor sites for conventional split thickness skin grafts are limited. In such cases, cultured epithelial (keratinocyte) grafts are prepared from small samples of the patient's own skin and expanded in tissue culture, a process that may incur very many cell divisions. Telomeres shorten with each cell division, and are markers of cellular proliferative history. We therefore measured telomere length in healed cultured epithelial autografts from four patients with burns, and noted that their telomeres were shorter than those in non-cultured skin from the same individuals, and than those in skin of healthy donors older than 80 years. Such great loss of telomeric DNA suggests that engrafted cells might have a shortened lifespan, which could have negative repercussions on the long-term viability of these grafts.

Full Text

Duke Authors

Cited Authors

  • Counter, CM; Press, W; Compton, CC

Published Date

  • April 19, 2003

Published In

Volume / Issue

  • 361 / 9366

Start / End Page

  • 1345 - 1346

PubMed ID

  • 12711471

International Standard Serial Number (ISSN)

  • 0140-6736

Digital Object Identifier (DOI)

  • 10.1016/S0140-6736(03)13042-5


  • eng

Conference Location

  • England