Human arteries engineered in vitro.

Journal Article (Journal Article)

There is a pressing need to develop methods to engineer small-calibre arteries for bypass surgery. We hypothesized that the rate-limiting step that has thwarted previous attempts to engineer such vessels from non-neonatal tissues is the limited proliferative capacity of smooth muscle cells (SMCs), which are the main cellular component of these vessels. Ectopic expression of the human telomerase reverse transcriptase subunit (hTERT) has been shown recently to extend the lifespan of certain human cells. We therefore introduced hTERT into human SMCs and found that the resulting cells proliferated far beyond their normal lifespan but retained characteristics of normal control SMCs. Importantly, using these non-neonatal SMCs, we were able to engineer mechanically robust human vessels, a crucial step towards creating arteries of clinical value for bypass surgery.

Full Text

Duke Authors

Cited Authors

  • McKee, JA; Banik, SSR; Boyer, MJ; Hamad, NM; Lawson, JH; Niklason, LE; Counter, CM

Published Date

  • June 2003

Published In

Volume / Issue

  • 4 / 6

Start / End Page

  • 633 - 638

PubMed ID

  • 12776184

Pubmed Central ID

  • PMC1319197

International Standard Serial Number (ISSN)

  • 1469-221X

Digital Object Identifier (DOI)

  • 10.1038/sj.embor.embor847


  • eng

Conference Location

  • England