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The nucleolar localization domain of the catalytic subunit of human telomerase.

Publication ,  Journal Article
Etheridge, KT; Banik, SSR; Armbruster, BN; Zhu, Y; Terns, RM; Terns, MP; Counter, CM
Published in: J Biol Chem
July 5, 2002

Telomerase is the enzyme essential to complete the replication of the terminal DNA of most eukaryotic chromosomes. In humans, this enzyme is composed of the telomerase reverse transcriptase (hTERT) and telomerase RNA (hTR) subunits. hTR has been found in the nucleolus, a site of assembly of ribosomes as well as other ribonucleoproteins (RNPs). We therefore tested whether the hTERT component is also found in the nucleolus, where it could complex with the hTR RNA to form a functional enzyme. We report here that hTERT does indeed localize to the nucleolus, and we mapped the domain responsible for this localization to the hTR-binding region of the protein by deletion analysis. Substitution mutations in two of the three conserved hTR-binding domains in this nucleolar localization domain (NoLD) abolished nucleolar localization. However, another mutation that impeded hTR binding did not alter this subcellular localization. Additionally, wild type hTERT was detected in the nucleolus of cells that failed to express hTR. Taken together, we propose that the nucleolar localization of hTERT involves more than just the association with the hTR subunit. Furthermore, the coincidental targeting of both the hTR and hTERT subunits to the nucleolus supports the premise that the assembly of telomerase occurs in the nucleolus.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 5, 2002

Volume

277

Issue

27

Start / End Page

24764 / 24770

Location

United States

Related Subject Headings

  • Uterine Cervical Neoplasms
  • Tumor Cells, Cultured
  • Telomerase
  • Recombinant Proteins
  • Protein Subunits
  • Osteosarcoma
  • Mutagenesis, Site-Directed
  • Humans
  • Female
  • DNA-Binding Proteins
 

Citation

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MLA
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Etheridge, K. T., Banik, S. S. R., Armbruster, B. N., Zhu, Y., Terns, R. M., Terns, M. P., & Counter, C. M. (2002). The nucleolar localization domain of the catalytic subunit of human telomerase. J Biol Chem, 277(27), 24764–24770. https://doi.org/10.1074/jbc.M201227200
Etheridge, Katherine T., Soma S. R. Banik, Blaine N. Armbruster, Yusheng Zhu, Rebecca M. Terns, Michael P. Terns, and Christopher M. Counter. “The nucleolar localization domain of the catalytic subunit of human telomerase.J Biol Chem 277, no. 27 (July 5, 2002): 24764–70. https://doi.org/10.1074/jbc.M201227200.
Etheridge KT, Banik SSR, Armbruster BN, Zhu Y, Terns RM, Terns MP, et al. The nucleolar localization domain of the catalytic subunit of human telomerase. J Biol Chem. 2002 Jul 5;277(27):24764–70.
Etheridge, Katherine T., et al. “The nucleolar localization domain of the catalytic subunit of human telomerase.J Biol Chem, vol. 277, no. 27, July 2002, pp. 24764–70. Pubmed, doi:10.1074/jbc.M201227200.
Etheridge KT, Banik SSR, Armbruster BN, Zhu Y, Terns RM, Terns MP, Counter CM. The nucleolar localization domain of the catalytic subunit of human telomerase. J Biol Chem. 2002 Jul 5;277(27):24764–24770.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 5, 2002

Volume

277

Issue

27

Start / End Page

24764 / 24770

Location

United States

Related Subject Headings

  • Uterine Cervical Neoplasms
  • Tumor Cells, Cultured
  • Telomerase
  • Recombinant Proteins
  • Protein Subunits
  • Osteosarcoma
  • Mutagenesis, Site-Directed
  • Humans
  • Female
  • DNA-Binding Proteins