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hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization.

Publication ,  Journal Article
Meyerson, M; Counter, CM; Eaton, EN; Ellisen, LW; Steiner, P; Caddle, SD; Ziaugra, L; Beijersbergen, RL; Davidoff, MJ; Liu, Q; Bacchetti, S ...
Published in: Cell
August 22, 1997

Telomerase, the ribonucleoprotein enzyme that elongates telomeres, is repressed in normal human somatic cells but is reactivated during tumor progression. We report the cloning of a human gene, hEST2, that shares significant sequence similarity with the telomerase catalytic subunit genes of lower eukaryotes. hEST2 is expressed at high levels in primary tumors, cancer cell lines, and telomerase-positive tissues but is undetectable in telomerase-negative cell lines and differentiated telomerase-negative tissues. Moreover, the message is up-regulated concomitant with the activation of telomerase during the immortalization of cultured cells and down-regulated during in vitro cellular differentiation. Taken together, these observations suggest that the induction of hEST2 mRNA expression is required for the telomerase activation that occurs during cellular immortalization and tumor progression.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

August 22, 1997

Volume

90

Issue

4

Start / End Page

785 / 795

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Testis
  • Telomerase
  • Sequence Alignment
  • RNA
  • Proteins
  • Protein Conformation
  • Molecular Sequence Data
 

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Meyerson, M., Counter, C. M., Eaton, E. N., Ellisen, L. W., Steiner, P., Caddle, S. D., … Weinberg, R. A. (1997). hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell, 90(4), 785–795. https://doi.org/10.1016/s0092-8674(00)80538-3
Meyerson, M., C. M. Counter, E. N. Eaton, L. W. Ellisen, P. Steiner, S. D. Caddle, L. Ziaugra, et al. “hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization.Cell 90, no. 4 (August 22, 1997): 785–95. https://doi.org/10.1016/s0092-8674(00)80538-3.
Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, et al. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell. 1997 Aug 22;90(4):785–95.
Meyerson, M., et al. “hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization.Cell, vol. 90, no. 4, Aug. 1997, pp. 785–95. Pubmed, doi:10.1016/s0092-8674(00)80538-3.
Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bacchetti S, Haber DA, Weinberg RA. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell. 1997 Aug 22;90(4):785–795.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

August 22, 1997

Volume

90

Issue

4

Start / End Page

785 / 795

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Testis
  • Telomerase
  • Sequence Alignment
  • RNA
  • Proteins
  • Protein Conformation
  • Molecular Sequence Data