The catalytic subunit of yeast telomerase.

Published

Journal Article

Telomerase is an RNA-directed DNA polymerase, composed of RNA and protein subunits, that replicates the telomere ends of linear eukaryotic chromosomes. Using a genetic strategy described here, we identify the product of the EST2 gene, Est2p, as a subunit of telomerase in the yeast Saccharomyces cerevisiae. Est2p is required for enzyme catalysis, as mutations in EST2 were found to result in the absence of telomerase activity. Immunochemical experiments show that Est2p is an integral subunit of the telomerase enzyme. Critical catalytic residues present in RNA-directed DNA polymerases are conserved in Est2p; mutation of one such residue abolishes telomerase activity, suggesting a direct catalytic role for Est2p.

Full Text

Duke Authors

Cited Authors

  • Counter, CM; Meyerson, M; Eaton, EN; Weinberg, RA

Published Date

  • August 19, 1997

Published In

Volume / Issue

  • 94 / 17

Start / End Page

  • 9202 - 9207

PubMed ID

  • 9256460

Pubmed Central ID

  • 9256460

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.17.9202

Language

  • eng

Conference Location

  • United States