Putative telomere-recruiting domain in the catalytic subunit of human telomerase.

Published

Journal Article

Telomerase, the enzyme that elongates telomeres, is essential to maintain telomere length and to immortalize most cancer cells. However, little is known about the regulation of this enzyme in higher eukaryotes. We previously described a domain in the hTERT telomerase catalytic subunit that is essential for telomere elongation and cell immortalization in vivo but dispensable for catalytic activity in vitro. Here, we show that fusions of hTERT containing different mutations in this domain to the telomere binding protein hTRF2 redirected the mutated hTERT to telomeres and rescued its in vivo functions. We suggest that this domain posttranscriptionally regulates telomerase function by targeting the enzyme to telomeres.

Full Text

Duke Authors

Cited Authors

  • Armbruster, BN; Etheridge, KT; Broccoli, D; Counter, CM

Published Date

  • May 2003

Published In

Volume / Issue

  • 23 / 9

Start / End Page

  • 3237 - 3246

PubMed ID

  • 12697823

Pubmed Central ID

  • 12697823

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.23.9.3237-3246.2003

Language

  • eng

Conference Location

  • United States