Putative telomere-recruiting domain in the catalytic subunit of human telomerase.
Journal Article (Journal Article)
Telomerase, the enzyme that elongates telomeres, is essential to maintain telomere length and to immortalize most cancer cells. However, little is known about the regulation of this enzyme in higher eukaryotes. We previously described a domain in the hTERT telomerase catalytic subunit that is essential for telomere elongation and cell immortalization in vivo but dispensable for catalytic activity in vitro. Here, we show that fusions of hTERT containing different mutations in this domain to the telomere binding protein hTRF2 redirected the mutated hTERT to telomeres and rescued its in vivo functions. We suggest that this domain posttranscriptionally regulates telomerase function by targeting the enzyme to telomeres.
Full Text
Duke Authors
Cited Authors
- Armbruster, BN; Etheridge, KT; Broccoli, D; Counter, CM
Published Date
- May 2003
Published In
Volume / Issue
- 23 / 9
Start / End Page
- 3237 - 3246
PubMed ID
- 12697823
Pubmed Central ID
- PMC153184
International Standard Serial Number (ISSN)
- 0270-7306
Digital Object Identifier (DOI)
- 10.1128/MCB.23.9.3237-3246.2003
Language
- eng
Conference Location
- United States