Activation of RalA is critical for Ras-induced tumorigenesis of human cells.

Published

Journal Article

RalGEFs were recently shown to be critical for Ras-mediated transformed and tumorigenic growth of human cells. We now show that the oncogenic activity of these proteins is propagated by activation of one RalGEF substrate, RalA, but blunted by another closely related substrate, RalB, and that the oncogenic signaling requires binding of the RalBP1 and exocyst subunit effector proteins. Knockdown of RalA expression impeded, if not abolished, the ability of human cancer cells to form tumors. RalA was also commonly activated in a panel of cell lines from pancreatic cancers, a disease characterized by activation of Ras. Activation of RalA signaling thus appears to be a critical step in Ras-induced transformation and tumorigenesis of human cells.

Full Text

Duke Authors

Cited Authors

  • Lim, K-H; Baines, AT; Fiordalisi, JJ; Shipitsin, M; Feig, LA; Cox, AD; Der, CJ; Counter, CM

Published Date

  • June 2005

Published In

Volume / Issue

  • 7 / 6

Start / End Page

  • 533 - 545

PubMed ID

  • 15950903

Pubmed Central ID

  • 15950903

Electronic International Standard Serial Number (EISSN)

  • 1878-3686

International Standard Serial Number (ISSN)

  • 1535-6108

Digital Object Identifier (DOI)

  • 10.1016/j.ccr.2005.04.030

Language

  • eng