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Activation of RalA is critical for Ras-induced tumorigenesis of human cells.

Publication ,  Journal Article
Lim, K-H; Baines, AT; Fiordalisi, JJ; Shipitsin, M; Feig, LA; Cox, AD; Der, CJ; Counter, CM
Published in: Cancer Cell
June 2005

RalGEFs were recently shown to be critical for Ras-mediated transformed and tumorigenic growth of human cells. We now show that the oncogenic activity of these proteins is propagated by activation of one RalGEF substrate, RalA, but blunted by another closely related substrate, RalB, and that the oncogenic signaling requires binding of the RalBP1 and exocyst subunit effector proteins. Knockdown of RalA expression impeded, if not abolished, the ability of human cancer cells to form tumors. RalA was also commonly activated in a panel of cell lines from pancreatic cancers, a disease characterized by activation of Ras. Activation of RalA signaling thus appears to be a critical step in Ras-induced transformation and tumorigenesis of human cells.

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Published In

Cancer Cell

DOI

ISSN

1535-6108

Publication Date

June 2005

Volume

7

Issue

6

Start / End Page

533 / 545

Location

United States

Related Subject Headings

  • rho GTP-Binding Proteins
  • ral Guanine Nucleotide Exchange Factor
  • ral GTP-Binding Proteins
  • Vesicular Transport Proteins
  • Transfection
  • RNA, Small Interfering
  • Proto-Oncogene Proteins p21(ras)
  • Protein Transport
  • Protein Binding
  • Pancreatic Neoplasms
 

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Lim, K.-H., Baines, A. T., Fiordalisi, J. J., Shipitsin, M., Feig, L. A., Cox, A. D., … Counter, C. M. (2005). Activation of RalA is critical for Ras-induced tumorigenesis of human cells. Cancer Cell, 7(6), 533–545. https://doi.org/10.1016/j.ccr.2005.04.030
Lim, Kian-Huat, Antonio T. Baines, James J. Fiordalisi, Michail Shipitsin, Larry A. Feig, Adrienne D. Cox, Channing J. Der, and Christopher M. Counter. “Activation of RalA is critical for Ras-induced tumorigenesis of human cells.Cancer Cell 7, no. 6 (June 2005): 533–45. https://doi.org/10.1016/j.ccr.2005.04.030.
Lim K-H, Baines AT, Fiordalisi JJ, Shipitsin M, Feig LA, Cox AD, et al. Activation of RalA is critical for Ras-induced tumorigenesis of human cells. Cancer Cell. 2005 Jun;7(6):533–45.
Lim, Kian-Huat, et al. “Activation of RalA is critical for Ras-induced tumorigenesis of human cells.Cancer Cell, vol. 7, no. 6, June 2005, pp. 533–45. Pubmed, doi:10.1016/j.ccr.2005.04.030.
Lim K-H, Baines AT, Fiordalisi JJ, Shipitsin M, Feig LA, Cox AD, Der CJ, Counter CM. Activation of RalA is critical for Ras-induced tumorigenesis of human cells. Cancer Cell. 2005 Jun;7(6):533–545.
Journal cover image

Published In

Cancer Cell

DOI

ISSN

1535-6108

Publication Date

June 2005

Volume

7

Issue

6

Start / End Page

533 / 545

Location

United States

Related Subject Headings

  • rho GTP-Binding Proteins
  • ral Guanine Nucleotide Exchange Factor
  • ral GTP-Binding Proteins
  • Vesicular Transport Proteins
  • Transfection
  • RNA, Small Interfering
  • Proto-Oncogene Proteins p21(ras)
  • Protein Transport
  • Protein Binding
  • Pancreatic Neoplasms