Altered localization of choline transporter sites in the mouse hippocampus after prenatal heroin exposure.

Published

Journal Article

Prenatal heroin exposure disrupts hippocampal cholinergic synaptic function and related behaviors. Biochemical studies indicate an increase in the number of presynaptic high-affinity choline transporter (HACT) sites, as assessed by [3H]hemicholinium-3 (HC-3) binding. The present study was designed to assess whether this effect involves global upregulation of the transporter, or whether disruption occurs with a specific tempero-spatial distribution. Pregnant mice were given 10mg/kg per day of heroin subcutaneously on gestational days (GD) 9-18. Autoradiographic distribution of HC-3 binding sites was evaluated in the hippocampus of the offspring at postnatal days 15, 25, and 53. These results, suggestive of hippocampal "miswiring," are likely to explain the net impairment of cholinergic synaptic function after prenatal heroin exposure, despite the simultaneous upregulation of both presynaptic cholinergic activity and postsynaptic receptors. Understanding the subregional selectivity of hippocampal defects can lead to the development of strategies that may potentially enable therapeutic interventions to offset or reverse the neurobehavioral defects.

Full Text

Duke Authors

Cited Authors

  • Vatury, O; Barg, J; Slotkin, TA; Yanai, J

Published Date

  • March 2004

Published In

Volume / Issue

  • 63 / 1

Start / End Page

  • 25 - 32

PubMed ID

  • 15121236

Pubmed Central ID

  • 15121236

Electronic International Standard Serial Number (EISSN)

  • 1873-2747

International Standard Serial Number (ISSN)

  • 0361-9230

Digital Object Identifier (DOI)

  • 10.1016/j.brainresbull.2003.11.004

Language

  • eng