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Perinatal environmental tobacco smoke exposure in rhesus monkeys: critical periods and regional selectivity for effects on brain cell development and lipid peroxidation.

Publication ,  Journal Article
Slotkin, TA; Pinkerton, KE; Seidler, FJ
Published in: Environ Health Perspect
January 2006

Perinatal environmental tobacco smoke (ETS) exposure in humans elicits neurobehavioral deficits. We exposed rhesus monkeys to ETS during gestation and through 13 months postnatally, or postnatally only (6-13 months). At the conclusion of exposure, we examined cerebrocortical regions and the midbrain for cell damage markers and lipid peroxidation. For perinatal ETS, two archetypal patterns were seen in the various regions, one characterized by cell loss (reduced DNA concentration) and corresponding increases in cell size (increased protein/DNA ratio), and a second pattern suggesting replacement of larger neuronal cells with smaller and more numerous glia (increased DNA concentration, decreased protein/DNA ratio). The membrane/total protein ratio, a biomarker of neurite formation, also indicated potential damage to neuronal projections, accompanied by reactive sprouting. When ETS exposure was restricted to the postnatal period, the effects were similar in regional selectivity, direction, and magnitude. These patterns resemble the effects of prenatal nicotine exposure in rodent and primate models. Surprisingly, perinatal ETS exposure reduced the level of lipid peroxidation as assessed by the concentration of thiobarbituric acid reactive species, whereas postnatal ETS did not. The heart, a tissue that, like the brain, has high oxygen demand, displayed a similar but earlier decrease (2-3 months) in lipid peroxidation in the perinatal exposure model, whereas values were reduced at 13 months with the postnatal exposure paradigm. Our results provide a mechanistic connection between perinatal ETS exposure and neurobehavioral anomalies, reinforce the role of nicotine in these effects, and buttress the importance of restricting or eliminating ETS exposure in young children.

Duke Scholars

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Published In

Environ Health Perspect

DOI

ISSN

0091-6765

Publication Date

January 2006

Volume

114

Issue

1

Start / End Page

34 / 39

Location

United States

Related Subject Headings

  • Toxicology
  • Tobacco Smoke Pollution
  • Thiobarbituric Acid Reactive Substances
  • Receptors, Adrenergic, beta
  • Receptor, Muscarinic M2
  • Proteins
  • Pregnancy
  • Membrane Proteins
  • Maternal Exposure
  • Male
 

Citation

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Slotkin, T. A., Pinkerton, K. E., & Seidler, F. J. (2006). Perinatal environmental tobacco smoke exposure in rhesus monkeys: critical periods and regional selectivity for effects on brain cell development and lipid peroxidation. Environ Health Perspect, 114(1), 34–39. https://doi.org/10.1289/ehp.8286
Slotkin, Theodore A., Kent E. Pinkerton, and Frederic J. Seidler. “Perinatal environmental tobacco smoke exposure in rhesus monkeys: critical periods and regional selectivity for effects on brain cell development and lipid peroxidation.Environ Health Perspect 114, no. 1 (January 2006): 34–39. https://doi.org/10.1289/ehp.8286.
Slotkin, Theodore A., et al. “Perinatal environmental tobacco smoke exposure in rhesus monkeys: critical periods and regional selectivity for effects on brain cell development and lipid peroxidation.Environ Health Perspect, vol. 114, no. 1, Jan. 2006, pp. 34–39. Pubmed, doi:10.1289/ehp.8286.

Published In

Environ Health Perspect

DOI

ISSN

0091-6765

Publication Date

January 2006

Volume

114

Issue

1

Start / End Page

34 / 39

Location

United States

Related Subject Headings

  • Toxicology
  • Tobacco Smoke Pollution
  • Thiobarbituric Acid Reactive Substances
  • Receptors, Adrenergic, beta
  • Receptor, Muscarinic M2
  • Proteins
  • Pregnancy
  • Membrane Proteins
  • Maternal Exposure
  • Male