Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism.

Published

Journal Article

Okadaic acid is a polyether derivative of 38-carbon fatty acid, and is implicated as the causative agent of diarrhetic shellfish poisoning. It is a potent tumour promoter that is not an activator of protein kinase C, but is a powerful inhibitor of protein phosphatases-1 and -2A (PP1 and PP2A) in vitro. We report here that okadaic acid rapidly stimulates protein phosphorylation in intact cells, and behaves like a specific protein phosphatase inhibitor in a variety of metabolic processes. Our results indicate that PP1 and PP2A are the dominant protein phosphatases acting on a wide range of phosphoproteins in vivo. We also find that okadaic acid mimics the effect of insulin on glucose transport in adipocytes, which suggests that this process is stimulated by a serine/threonine phosphorylation event.

Full Text

Duke Authors

Cited Authors

  • Haystead, TA; Sim, AT; Carling, D; Honnor, RC; Tsukitani, Y; Cohen, P; Hardie, DG

Published Date

  • January 5, 1989

Published In

Volume / Issue

  • 337 / 6202

Start / End Page

  • 78 - 81

PubMed ID

  • 2562908

Pubmed Central ID

  • 2562908

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/337078a0

Language

  • eng

Conference Location

  • England