PHAS-I as a link between mitogen-activated protein kinase and translation initiation.

Published

Journal Article

PHAS-I is a heat-stable protein (relative molecular mass approximately 12,400) found in many tissues. It is rapidly phosphorylated in rat adipocytes incubated with insulin or growth factors. Nonphosphorylated PHAS-I bound to initiation factor 4E (eIF-4E) and inhibited protein synthesis. Serine-64 in PHAS-I was rapidly phosphorylated by mitogen-activated (MAP) kinase, the major insulin-stimulated PHAS-I kinase in adipocyte extracts. Results obtained with antibodies, immobilized PHAS-I, and a messenger RNA cap affinity resin indicated that PHAS-I did not bind eIF-4E when serine-64 was phosphorylated. Thus, PHAS-I may be a key mediator of the stimulation of protein synthesis by the diverse group of agents and stimuli that activate MAP kinase.

Full Text

Duke Authors

Cited Authors

  • Lin, TA; Kong, X; Haystead, TA; Pause, A; Belsham, G; Sonenberg, N; Lawrence, JC

Published Date

  • October 28, 1994

Published In

Volume / Issue

  • 266 / 5185

Start / End Page

  • 653 - 656

PubMed ID

  • 7939721

Pubmed Central ID

  • 7939721

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.7939721

Language

  • eng

Conference Location

  • United States